Valen, Eivind and Preker, Pascal and Andersen, Peter Refsing and Zhao, Xiaobei and Chen, Yun and Ender, Christine and Dueck, Anne and Meister, Gunter and Sandelin, Albin and Jensen, Torben Heick (2011) Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 18 (9). 1075-U1702. ISSN 1545-9993, 1545-9985
Full text not available from this repository. (Request a copy)Abstract
Efforts to catalog eukaryotic transcripts have uncovered many small RNAs (sRNAs) derived from gene termini and splice sites. Their biogenesis pathways are largely unknown, but a mechanism based on backtracking of RNA polymerase II (RNAPII) has been suggested. By sequencing transcripts 12-100 nucleotides in length from cells depleted of major RNA degradation enzymes and RNAs associated with Argonaute (AGO1/2) effector proteins, we provide mechanistic models for sRNA production. We suggest that neither splice site-associated (SSa) nor transcription start site-associated (TSSa) RNAs arise from RNAPII backtracking. Instead, SSa RNAs are largely degradation products of splicing intermediates, whereas TSSa RNAs probably derive from nascent RNAs protected by stalled RNAPII against nucleolysis. We also reveal new AGO1/2-associated RNAs derived from 3' ends of introns and from mRNA 3' UTRs that appear to draw from noncanonical microRNA biogenesis pathways.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSCRIPTION INITIATION; DIVERGENT TRANSCRIPTION; PERVASIVE TRANSCRIPTION; MICRORNA BIOGENESIS; ARGONAUTE PROTEINS; REGULATORY RNAS; TINY RNAS; POLYMERASE; ELONGATION; PROMOTERS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2020 06:32 |
| Last Modified: | 27 Jan 2021 12:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20324 |
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