Mark, Jonas Josef Peter and Coufal, Pavel and Opekar, Frantisek and Matysik, Frank-Michael (2011) Comparison of the performance characteristics of two tubular contactless conductivity detectors with different dimensions and application in conjunction with HPLC. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 401 (5). pp. 1669-1676. ISSN 1618-2642,
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Two tubular capacitively coupled contactless conductivity detection (C(4)D) cells with different geometric dimensions were evaluated with regard to their main analytical characteristics under non-separation and separation conditions in conjunction with liquid chromatography. A comparison of the performance of the tubular cells to a previously tested thin-layer detection cell was drawn. Additionally, using a theoretical model the experimental results were compared with sets of calculated values and partially enabled to model the complex behavior of C(4)D detection in combination with high-performance liquid chromatography (HPLC). While cell 1 is characterized by a geometric cell volume of 0.6 mu L, a wall thickness of 675 mu m, and an inner diameter of 125 mu m, the respective values for cell 2 are 2.3 mu L, 200 mu m, and 250 mu m. The main analytical parameters were evaluated using a potassium chloride (KCl) solution. The limits of detection were 0.4 mu M KCl (5.7 x 10(-6) S m(-1)) for cell 1 and 0.2 mu M KCl (3.2 x 10(-6) S m(-1)) for cell 2, which compares well to the previously found 0.2 mu M for the thin-layer cell. A pair of linear ranges was found for both cells in a concentration interval ranging from 1 x 10(-6) to 1 x 10(-4) M (corresponding to 1.5 x 10(-5) to 1.5 x 10(-3) S m(-1)) KCl, respectively. Furthermore, the detector cells were applied to the HPLC separation of a model compound system consisting of benzoic acid, lactic acid, octanesulphonic acid, and sodium capronate. Separation of the compounds was achieved with a Biospher PSI 100 C18 column using 60% aqueous acetonitrile mobile phase. Calibration curves for the examined model system were well correlated (rA(2)aEuro parts per thousand > 0.997), and it was found that under separation conditions the arrangement with the lower cell volume (cell 1) yields higher sensitivity and respectively lower limits of detection for all model compounds. Compared with the thin-layer cell, the tubular cells show better overall performance in regard to the determined analytical characteristics.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICROCHIP CAPILLARY-ELECTROPHORESIS; LIQUID-CHROMATOGRAPHY; INORGANIC-IONS; CELL GEOMETRY; FREQUENCY; PEPTIDES; COLUMN; ACIDS; Contactless conductivity detection; Flow analysis; Electrochemical detection; Detection cell geometry; Liquid chromatography |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2020 10:15 |
| Last Modified: | 02 Jun 2020 10:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20345 |
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