Saleh, Sayed M. and Ali, Reham and Wolfbeis, Otto S. (2011) New silica and polystyrene nanoparticles labeled with longwave absorbing and fluorescent chameleon dyes. MICROCHIMICA ACTA, 174 (3-4). pp. 429-434. ISSN 0026-3672, 1436-5073
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We are presenting new fluorescent nanoparticles (NPs) made from silica or polystyrene. Such NPs are potentially useful for purposes of cellular imaging and sensing. The NPs were surface-modified with amino groups, and longwave absorbing and emitting dyes were then conjugated, via their reactive chloro atoms, to the NPs. The reactions proceed at temperatures of around 65 degrees C and in predominantly aqueous solution, and are accompanied by a color change from typically green to blue. By analogy to other labels giving this effect, we refer to such dyes as chameleon labels. All NPs were characterized in terms of size, by absorption and emission spectroscopy, thermogravimetry and zeta potentials. The chameleon effect also was used to detect the presence of minute quantities of amino groups on the surface of NPs, both by absorptiometry and, with particular sensitivity, by fluorescence.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BIOMOLECULES; LASER; RED; PH; Nanoparticles; Silica; Polystyrene; Fluorescence; Label |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Jun 2020 05:08 |
| Last Modified: | 03 Jun 2020 05:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20355 |
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