Kremer, Werner and Arnold, Martin and Munte, Claudia Elisabeth and Hartl, Rainer and Erlach, Markus Beck and Koehler, Joerg and Meier, Alexander and Kalbitzer, Hans Robert (2011) Pulsed Pressure Perturbations, an Extra Dimension in NMR Spectroscopy of Proteins. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133 (34). pp. 13646-13651. ISSN 0002-7863,
Full text not available from this repository. (Request a copy)Abstract
The introduction of multidimensional NMR spectroscopy was a breakthrough in biological NMR methodology because it allowed the unequivocal correlation of different spin states of the system. The introduction of large pressure perturbations in the corresponding radio frequency (RF) pulse sequences adds an extra structural dimension into these experiments. We have developed a microprocessor-controlled pressure jump unit that is able to introduce fast, strong pressure changes at any point in the pulse sequences. Repetitive pressure changes of 80 MPa in the sample tube are thus feasible in less than 30 ms. Two general forms of these experiments are proposed here, the pressure perturbation transient state spectroscopy (PPTSS) and the pressure perturbation state correlation spectroscopy (PPSCS). PPTSS can be used to measure the rate constants and the activation energies and activation volumes for the transition between different conformational states including the folded and unfolded state of proteins, for polymerization-depolymerization processes, and for ligand binding at atomic resolution. PPSCS spectroscopy correlates the NMR parameters of different pressure-induced states of the system, thus allowing the measurement of properties of a given pressure induced state such as a folding intermediate in a different state, for example, the folded state. Selected examples for PPTSS and PPSCS spectroscopy are presented in this Article.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CONTAINING PHOSPHOCARRIER PROTEIN; AQUEOUS-SOLUTIONS; SENSITIVITY; SUPPRESSION; RELAXATION; EXCHANGE; SPECTRA; C-13; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2020 08:15 |
| Last Modified: | 02 Jun 2020 08:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20376 |
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