Boy, Sandra and Sauerbruch, Sophie and Kraemer, Mathias and Schormann, Thorsten and Schlachetzki, Felix and Schuierer, Gerhard and Luerding, Ralph and Hennemann, Burkhard and Orso, Evelyn and Dabringhaus, Andreas and Winkler, Juergen and Bogdahn, Ulrich (2011) Mobilisation of Hematopoietic CD34(+) Precursor Cells in Patients with Acute Stroke Is Safe - Results of an Open-Labeled Non Randomized Phase I/II Trial. PLOS ONE, 6 (8): e23099. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Background: Regenerative strategies in the treatment of acute stroke may have great potential. Hematopoietic growth factors mobilize hematopoietic stem cells and may convey neuroprotective effects. We examined the safety, potential functional and structural changes, and CD34(+) cell-mobilization characteristics of G-CSF treatment in patients with acute ischemic stroke. Methods and Results: Three cohorts of patients (8, 6, and 6 patients per cohort) were treated subcutaneously with 2.5, 5, or 10 mu g/kg body weight rhG-CSF for 5 consecutive days within 12 hrs of onset of acute stroke. Standard treatment included IV thrombolysis. Safety monitoring consisted of obtaining standardized clinical assessment scores, monitoring of CD34(+) stem cells, blood chemistry, serial neuroradiology, and neuropsychology. Voxel-guided morphometry (VGM) enabled an assessment of changes in the patients' structural parenchyma. 20 patients (mean age 55 yrs) were enrolled in this study, 5 of whom received routine thrombolytic therapy with r-tPA. G-CSF treatment was discontinued in 4 patients because of unrelated adverse events. Mobilization of CD34(+) cells was observed with no concomitant changes in blood chemistry, except for an increase in the leukocyte count up to 75,500/mu l. Neuroradiological and neuropsychological follow-up studies did not disclose any specific G-CSF toxicity. VGM findings indicated substantial atrophy of related hemispheres, a substantial increase in the CSF space, and a localized increase in parenchyma within the ischemic area in 2 patients. Conclusions: We demonstrate a good safety profile for daily administration of G-CSF when begun within 12 hours after onset of ischemic stroke and, in part in combination with routine IV thrombolysis. Additional analyses using VGM and a battery of neuropsychological tests indicated a positive functional and potentially structural effect of G-CSF treatment in some of our patients.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONY-STIMULATING FACTOR; FOCAL CEREBRAL-ISCHEMIA; VOXEL-GUIDED MORPHOMETRY; FACTOR G-CSF; BONE-MARROW; FUNCTIONAL RECOVERY; PERIPHERAL-BLOOD; STEM-CELLS; WORKING-MEMORY; INFARCT VOLUME; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Medicine > Lehrstuhl für Neurologie Medicine > Lehrstuhl für Röntgendiagnostik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Jun 2020 05:13 |
| Last Modified: | 03 Jun 2020 05:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20383 |
Actions (login required)
 |
View Item |
