Penzkofer, Alfons and Stierl, M. and Hegemann, P. and Kateriya, Suneel (2011) Photo-dynamics of the BLUF domain containing soluble adenylate cyclase (nPAC) from the amoeboflagellate Naegleria gruberi NEG-M strain. CHEMICAL PHYSICS, 387 (1-3). pp. 25-38. ISSN 0301-0104,
Full text not available from this repository. (Request a copy)Abstract
The amoeboflagellate Naegleria gruberi NEG-M comprises a BLUF (blue light sensor using flavin) regulated adenylate cyclase (nPAC). The nPAC gene was expressed heterologously in Escherichia coli and the photodynamics of the nPAC protein was studied by optical absorption and fluorescence spectroscopy. Bluelight exposure of nPAC caused a typical BLUF-type photo-cycle behavior (spectral absorption red-shift, fluorescence quenching, absorption and fluorescence recovery in the dark). Additionally, time-delayed reversible photo-induced one-electron reduction of fully oxidized flavin (Fl(ox)) to semi-reduced flavin (FlH(.)) occurred. Furthermore, photo-excitation of FlH(.) caused irreversible electron transfer to fully reduced anionic flavin (FlH(center dot)). A photo-induced electron transfer from Tyr or Trp to flavin (Tyr(+)-Fl(center dot) or Trp(center dot+)- Fl(center dot)radical anion-Fl radical anion or Tyr radical anion-Fl radical anion radical ion-pair formation) is thought to cause H-bond restructuring responsible for BLUF-type photo-cycling and permanent protein re-conformation enabling photo-induced flavin reduction by proton transfer. Some photo-degradation of Flox to lumichrome was observed. A model of the photo-dynamics of nPAC is developed. (C) 2011 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLUORESCENCE SPECTROSCOPIC CHARACTERIZATION; LIGHT-REGULATED PHOSPHODIESTERASE; MEDIATED SIGNAL-TRANSDUCTION; FLAGELLATE EUGLENA-GRACILIS; SYNECHOCYSTIS SP PCC6803; RHODOBACTER-SPHAEROIDES; CHLAMYDOMONAS-REINHARDTII; INFRARED-SPECTROSCOPY; FLAVIN MONONUCLEOTIDE; CRYSTAL-STRUCTURES; BLUF domain; Cyclase homology domain; Amoeboflagellate Naegleria gruberi; BLUF-type photo-cycle; One-electron reduction-oxidation photocycle; Photo-degradation; Flavin; Flavin-semiquinone; Flavin-hydroquinone; Flavin anion radical |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Jun 2020 05:15 |
| Last Modified: | 03 Jun 2020 05:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20384 |
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