Stolwijk, Judith A. and Hartmann, Christoph and Balani, Poonam and Albermann, Silke and Keese, Charles R. and Giaever, Ivar and Wegener, Joachim (2011) Impedance analysis of adherent cells after in situ electroporation: Non-invasive monitoring during intracellular manipulations. BIOSENSORS & BIOELECTRONICS, 26 (12). pp. 4720-4727. ISSN 0956-5663,
Full text not available from this repository. (Request a copy)Abstract
In this study adherent animal cells were grown to confluence on circular gold-film electrodes of 250 mu m diameter that had been deposited on the surface of a regular culture dish. The impedance of the cell-covered electrode was measured at designated frequencies to monitor the behavior of the cells with time. This approach is referred to as electric cell-substrate impedance sensing or short ECIS in the literature. The gold-film electrodes were also used to deliver well-defined AC voltage pulses of several volts amplitude and several hundred milliseconds duration to the adherent cells in order to achieve reversible membrane electroporation (in situ electroporation = ISE). Electroporation-assisted introduction of membrane impermeable molecules into the cytoplasm was studied by using FITC-labeled dextran molecules of different molecular weights. Probes as big as 2 MDa were successfully loaded into the cells residing on the electrode surface. Time-resolved impedance measurements before and immediately after the electroporation pulse revealed the kinetics of membrane resealing as well as subsequent changes in cell morphology. Cells recovered from the electroporation pulse within less than 90 min. When membrane-impermeable, bioactive compounds like N-3(-) or bleomycin were introduced into the cells by in situ electroporation, concomitant ECIS readings sensitively reported on the associated response of the cells to these toxins as a function of time (ISE-ECIS). (C) 2011 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAMMALIAN-CELLS; ELECTROPERMEABILIZATION; BLEOMYCIN; MEMBRANE; VITRO; DNA; MACROMOLECULES; DENATURATION; CYTOTOXICITY; TRANSFECTION; In situ electroporation(ISE); Electric cell-substrate impedance sensing (ECIS); Whole cell biosensors; Impedmetric monitoring; Bleomycin |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2020 09:13 |
| Last Modified: | 02 Jun 2020 09:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20396 |
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