Giannone, Richard J. and Huber, Harald and Karpinets, Tatiana and Heimerl, Thomas and Kueper, Ulf and Rachel, Reinhard and Keller, Martin and Hettich, Robert L. and Podar, Mircea (2011) Proteomic Characterization of Cellular and Molecular Processes that Enable the Nanoarchaeum equitans-Ignicoccus hospitalis Relationship. PLOS ONE, 6 (8): e22942. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Nanoarchaeum equitans, the only cultured representative of the Nanoarchaeota, is dependent on direct physical contact with its host, the hyperthermophile Ignicoccus hospitalis. The molecular mechanisms that enable this relationship are unknown. Using whole-cell proteomics, differences in the relative abundance of >75% of predicted protein-coding genes from both Archaea were measured to identify the specific response of I. hospitalis to the presence of N. equitans on its surface. A purified N. equitans sample was also analyzed for evidence of interspecies protein transfer. The depth of cellular proteome coverage achieved here is amongst the highest reported for any organism. Based on changes in the proteome under the specific conditions of this study, I. hospitalis reacts to N. equitans by curtailing genetic information processing (replication, transcription) in lieu of intensifying its energetic, protein processing and cellular membrane functions. We found no evidence of significant Ignicoccus biosynthetic enzymes being transported to N. equitans. These results suggest that, under laboratory conditions, N. equitans diverts some of its host's metabolism and cell cycle control to compensate for its own metabolic shortcomings, thus appearing to be entirely dependent on small, transferable metabolites and energetic precursors from I. hospitalis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SET ENRICHMENT ANALYSIS; QUANTITATIVE PROTEOMICS; SHOTGUN PROTEOMICS; MASS-SPECTROMETRY; OUTER-MEMBRANE; PROTEIN; ARCHAEA; INSIGHTS; GENOME; DATABASE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Jun 2020 06:04 |
| Last Modified: | 03 Jun 2020 06:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20410 |
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