Baumgarten, S. and Robelek, R. (2011) Surface plasmon resonance (SPR) sensors for the rapid, sensitive detection of the cellular response to osmotic stress. SENSORS AND ACTUATORS B-CHEMICAL, 156 (2). pp. 798-804. ISSN 0925-4005,
Full text not available from this repository. (Request a copy)Abstract
Cells regulate their volume in response to changes in osmolarity of both, their extracellular and intracellular environments. As stability of the cell volume is a compelling exigency for cellular integrity, techniques for a sensitive, time-resolved volume measurement of adherently grown mammalian cells attract considerable interest, especially in the field of cell physiology and biology. In this study we apply a surface plasmon resonance (SPR) based biosensor for the comparative analysis of the volume responses of two renal epithelial cell types to non-isotonic challenges. The on-line, label-free and non-invasive biosensor format shows distinct similarities and differences in the reaction kinetics of the two cell types. Furthermore regulatory volume responses to the osmotic stimuli as well as their inhibition by Gd3+ ions can be observed with a high time-resolution. Limit-of-detection measurements indicate the high sensitivity of the sensor capable of detecting cellular volume responses of adherently grown mammalian cells to osmotic stimuli well below a bioanalytical relevant value of 5 mOsm/kg. (C) 2011 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VOLUME REGULATION; WATER PERMEABILITY; HYPERTONIC SALINE; EPITHELIAL-CELLS; MICROSCOPY; MECHANISMS; ION; MYOCYTES; CULTURE; Surface plasmon resonance; Biosensor; Osmotic stress; Cellular reaction; Cell volume change |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jun 2020 04:54 |
| Last Modified: | 05 Jun 2020 04:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20511 |
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