Wadhier, Magdalena C. and Almstetter, Martin F. and Nuernberger, Nadine and Gruber, Michael A. and Dettmer, Katja and Oefner, Peter J. (2011) Improved enantiomer resolution and quantification of free D-amino acids in serum and urine by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 1218 (28). pp. 4537-4544. ISSN 0021-9673,
Full text not available from this repository. (Request a copy)Abstract
The potential of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC x GC-TOFMS) in the quantitative analysis of amino acid enantiomers (AAEs) as their methyl chloroformate (MCF) derivatives in physiological fluids was investigated. Of the two column sets tested, the combination of an Rt-gamma DEXsa chiral column with a polar ZB-AAA column provided superior selectivity. Twenty AAEs were baseline resolved including L-Leu and D-Ile, which had failed separation by one-dimensional chiral GC-quadrupole-MS (GC-qMS). Lower limits of quantification (LLOQ) were in the range of 0.03-2 mu M. Reproducibility of the analysis of a serum specimen in octaplicate ranged from 1.3 to 16.6%. The GC x GC-TOFMS method was validated by analyzing AAEs in 48 urine and 43 serum specimens, respectively, and by comparing the results with data obtained by a previously validated GC-qMS method. Mean recoveries ranged from 78.4% for D-Leu to 116.4% for D-Pro in urine and 72.2% for L-Thr to 129.4% for L-Ile in serum. The method was applied to the comparison of AAE serum levels in patients suffering from liver cirrhosis to a control group. Significantly increased D-AA concentrations were found for the patient group, whereas L-AA levels were slightly decreased. (C) 2011 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GC-MS; MICROBIOLOGY; NUTRITION; OXIDASE; ORIGIN; PLASMA; FLUIDS; Enantioselective analysis; GC x GC-TOFMS; Methyl chloroformate; Metabolomics; Urine; Serum |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jun 2020 07:21 |
| Last Modified: | 05 Jun 2020 07:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20537 |
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