Braig, S. and Wallner, S. and Junglas, B. and Fuchshofer, R. and Bosserhoff, A-K (2011) CTGF is overexpressed in malignant melanoma and promotes cell invasion and migration. BRITISH JOURNAL OF CANCER, 105 (2). pp. 231-238. ISSN 0007-0920,
Full text not available from this repository. (Request a copy)Abstract
BACKGROUND: Malignant melanoma cells are known to have altered expression of growth factors compared with normal human melanocytes. These changes most likely favour tumour growth and progression, and influence tumour environment. The induction of transforming growth factor beta1, 2 and 3 as well as BMP4 and BMP7 expression in malignant melanoma has been reported before, whereas the expression of an important modulator of these molecules, connective tissue growth factor (CTGF), has not been investigated in melanomas until now. METHODS: Expression of CTGF was analysed in melanoma cell lines and tissue samples by qRT-PCR and immunohistochemistry. To determine the regulation of CTGF expression in malignant melanoma, specific siRNA was used. Additionally, migration, invasion and attachment assays were carried out. RESULTS: We were able to demonstrate that CTGF expression is upregulated in nine melanoma cell lines and in primary and metastatic melanoma in situ. The transcription factor HIF-1 alpha was revealed as a positive regulator for CTGF expression. Melanoma cells, in which CTGF expression is diminished, show a strong reduction of migratory and invasive properties when compared with controls. Further, treatment of normal human epidermal melanocytes with recombinant CTGF leads to an increase of migratory and invasive behaviour of these cells. CONCLUSION: These results suggest that CTGF promotes melanoma cell invasion and migration and, therefore, has an important role in the progression of malignant melanoma. British Journal of Cancer (2011) 105, 231-238. doi: 10.1038/bjc.2011.226 www.bjcancer.com Published online 14 June 2011 (C) 2011 Cancer Research UK
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TISSUE-GROWTH-FACTOR; BONE MORPHOGENETIC PROTEIN-7; TRABECULAR MESHWORK CELLS; FACTOR-BETA; EXPRESSION; CANCER; PROLIFERATION; ANGIOGENESIS; MECHANISMS; RESISTANCE; malignant melanoma; CTGF; transcriptional regulation; invasion; hypoxia |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie Biology, Preclinical Medicine > Institut für Anatomie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jun 2020 07:25 |
| Last Modified: | 05 Jun 2020 07:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20541 |
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