From protein catalogues towards targeted proteomics approaches in cereal grains

Finnie, Christine and Sultan, Abida and Grasser, Klaus D. (2011) From protein catalogues towards targeted proteomics approaches in cereal grains. PHYTOCHEMISTRY, 72 (10). pp. 1145-1153. ISSN 0031-9422

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Abstract

Due to their importance for human nutrition, the protein content of cereal grains has been a subject of intense study for over a century and cereal grains were not surprisingly one of the earliest subjects for 2D-gel-based proteome analysis. Over the last two decades, countless cereal grain proteomes, mostly derived using 2D-gel based technologies, have been described and hundreds of proteins identified. However, very little is still known about post-translational modifications, subcellular proteomes, and protein-protein interactions in cereal grains. Development of techniques for improved extraction, separation and identification of proteins and peptides is facilitating functional proteomics and analysis of sub-proteomes from small amounts of starting material, such as seed tissues. The combination of proteomics with structural and functional analysis is increasingly applied to target subsets of proteins. These "next-generation" proteomics studies will vastly increase our depth of knowledge about the processes controlling cereal grain development, nutritional and processing characteristics. (C) 2010 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: WHEAT TRITICUM-AESTIVUM; POLYACRYLAMIDE-GEL-ELECTROPHORESIS; IMMOBILIZED PH GRADIENTS; BARLEY CULTIVAR DISCRIMINATION; GRANULE-ASSOCIATED PROTEINS; THIOREDOXIN-H ISOFORMS; RICE ORYZA-SATIVA; XYLANASE INHIBITOR; MAIZE ENDOSPERM; HMGB PROTEINS; Laser capture microdissection; Membrane proteins; Amyloplasts; Chromosomal proteins; Redox regulation; Xylanase inhibitors
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser)
Depositing User: Petra Gürster
Date Deposited: 30 Apr 2020 08:58
Last Modified: 30 Apr 2020 08:58
URI: https://pred.uni-regensburg.de/id/eprint/20592

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