Pawar, Kiran and Mueller, Rainer and Caioni, Massimiliano and Prang, Peter and Bogdahn, Ulrich and Kunz, Werner and Weidner, Norbert (2011) Increasing capillary diameter and the incorporation of gelatin enhance axon outgrowth in alginate-based anisotropic hydrogels. ACTA BIOMATERIALIA, 7 (7). pp. 2826-2834. ISSN 1742-7061,
Full text not available from this repository. (Request a copy)Abstract
Substantial recovery of function following peripheral and central nervous system (CNS) injury critically depends on longitudinally directed axon regeneration across the injury site, which requires a mechanical guidance providing scaffold. We have previously shown that anisotropic alginate-based hydrogels with a defined capillary diameter (25 mu m), which form via a self-organizing process driven by unidirectional diffusion of divalent cations into sodium alginate sols, promoted longitudinally oriented elongation of CNS axons in vitro and in vivo. In the present study the influence of various capillary diameters and the incorporation of gelatin to promote directed axon outgrowth and Schwann cell migration were assessed in a dorsal root ganglion outgrowth assay in vitro. Superimposing an alginate sol with Cu(2+), Sr(2+), or Zn(2+) ion containing solutions allowed the creation of hydrogels with capillaries 18, 25 and 55 mu m in diameter, respectively. Axon outgrowth and Schwann cell migration were analyzed in terms of axon length/density and Schwann cell density within the capillary structures. Axon ingrowth into capillary hydrogels, which was always accompanied by Schwann cells, was enhanced with increasing capillary diameter. The incorporation of gelatin did not influence overall axon density, but promoted the length of axon outgrowth within the hydrogels. The longitudinal orientation of axons decreased in wider capillaries, which suggests that medium-sized capillaries are the optimal substrate to elicit substantial axon growth and longitudinal orientation after axon injury. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | PERIPHERAL-NERVE REGENERATION; INJURED SPINAL-CORD; AGAROSE SCAFFOLDS; CELL-GROWTH; GUIDANCE; REPAIR; CHANNELS; COLLAGEN; MICROCHANNELS; STRATEGIES; Alginate hydrogel; Self-organization; Microchannels; Axon regeneration; Dorsal root ganglion |
Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
Divisions: | Medicine > Lehrstuhl für Neurologie Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz |
Depositing User: | Dr. Gernot Deinzer |
Date Deposited: | 08 Jun 2020 11:32 |
Last Modified: | 08 Jun 2020 11:32 |
URI: | https://pred.uni-regensburg.de/id/eprint/20605 |
Actions (login required)
View Item |