Sing, Andreas and Berger, Anja and Schneider-Brachert, Wulf and Holzmann, Thomas and Reischl, Udo (2011) Rapid Detection and Molecular Differentiation of Toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans Strains by LightCycler PCR. JOURNAL OF CLINICAL MICROBIOLOGY, 49 (7). pp. 2485-2489. ISSN 0095-1137,
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The systemic symptoms of diphtheria are caused by the tox-encoded diphtheria toxin (DT) which is produced by toxigenic Corynebacterium spp. Besides the classical agent C. diphtheriae, the zoonotic pathogen C. ulcerans has increasingly been reported as an emerging pathogen for diphtheria. The reliable detection of toxigenic Corynebacterium spp. is of substantial importance for both diphtheria surveillance in the public health sector and the clinical workup of a patient with diphtherialike symptoms. Since the respective tox genes of C. diphtheriae and C. ulcerans differ from each other in both DNA and amino acid sequence, both tox genes should be covered by novel real-time PCR methods. We describe the development and validation of a LightCycler PCR assay which reliably recognizes tox genes from both C. diphtheriae and C. ulcerans and differentiates the respective target genes by fluorescence resonance energy transfer (FRET) hybridization probe melting curve analysis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REAL-TIME PCR; TOXIN GENE; ILLNESS; ASSAY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jun 2020 05:25 |
| Last Modified: | 09 Jun 2020 05:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20615 |
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