Schaeferling, Michael and Groegel, Dominik B. M. and Schreml, Stephan (2011) Luminescent probes for detection and imaging of hydrogen peroxide. MICROCHIMICA ACTA, 174 (1-2). pp. 1-18. ISSN 0026-3672
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The relevance of hydrogen peroxide (H2O2) in biological processes has been underestimated for a long time. In recent years, various reports showed that H2O2 not only acts as a cytotoxic compound appearing in the course of oxidative stress, but also functions as an important signaling molecule. Fluorescent probes (or indicators) and nanoparticles that respond selectively to hydrogen peroxide can be applied for intracellular measurements or in vivo imaging, and are superior to electrochemical methods, e.g. in terms of spatial resolution. In contrast to previous reviews that concentrated on the adoption of different probes for certain applications, this survey highlights the basic principles of different probes in terms of their chemical design, structures and functionalities. Thus, the probes are classified according to the underlying reaction mechanism: oxidation, hydrolysis, photoinduced electron transfer, and lanthanide complexation. Other assays are based on fluorescent proteins and nanoparticles, and chemi- or bioluminescent reagents. We confine this review to probes that display a more or less distinct selectivity to hydrogen peroxide. Indicators responding to reactive oxygen species (ROS) in general, or to particular other ROS, are not covered. Finally, we briefly discuss future trends and perspectives of these luminescent reporters in biomedical research and imaging.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOTOINDUCED ELECTRON-TRANSFER; FLUORESCENT PROTEIN INDICATORS; OXIDATIVE STRESS; PEROXYOXALATE CHEMILUMINESCENCE; HORSERADISH-PEROXIDASE; GROWTH-FACTOR; LIVING CELLS; NITRIC-OXIDE; FLUOROMETRIC-DETERMINATION; ENDOTHELIAL-CELLS; Hydrogen peroxide; Reactive oxygen species; Fluorescent probes; Fluorescence imaging; Cell signaling |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Petra Gürster |
| Date Deposited: | 30 Apr 2020 10:27 |
| Last Modified: | 30 Apr 2020 10:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20621 |
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