Haselberger, Martina and Springwald, Anette and Konwisorz, Anna and Lattrich, Claus and Goerse, Regina and Ortmann, Olaf and Treeck, Oliver (2011) Silencing of the icb-1 gene inhibits the induction of differentiation-associated genes by vitamin D3 and all-trans retinoic acid in gynecological cancer cells. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 28 (1). pp. 121-127. ISSN 1107-3756, 1791-244X
Full text not available from this repository. (Request a copy)Abstract
Icb-1 (Clorf38) is a human gene initially described by our group to be involved in differentiation processes of cancer cells. To further elucidate the function of the icb-1 gene in differentiation of breast and endometrial cancer cells, we knocked down its expression by means of shRNA transfection. Knockdown of icb-1 inhibited the vitamin D-3-induced up-regulation of E-cadherin expression in both MCF-7 and HEC-1B cells. Induction of E-cadherin expression by all-trans retinoic acid (ATRA) was also blocked in both cell lines expressing icb-1 siRNA. Examination of icb-1 and E-cadherin expression in 66 breast cancer tissue samples revealed a significant positive correlation between the two genes. In MCF-7 cells, silencing of the icb-1 gene inhibited the ATRA and the vitamin D-3-induced up-regulation of lactoferrin and estrogen receptor beta expression. The data of our knockdown study suggest that icb-1 may act as a mediator of differentiation signals in breast cancer cells induced by ATRA or vitamin D-3. These findings together with the observed co-expression of icb-1 with E-cadherin in breast cancer samples support an important role of the icb-1 gene in cancer cell differentiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | E-CADHERIN; POSITIVE SELECTION; BREAST; EXPRESSION; LACTOFERRIN; ADHESION; OVARIAN; LINES; CARCINOMA; APOPTOSIS; Clorf38 gene; icb-1 gene; differentiation; cancer cell line; breast cancer |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jun 2020 06:22 |
| Last Modified: | 09 Jun 2020 06:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20634 |
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