Mortensen, Simon A. and Sonderkaer, Mads and Lynggaard, Carina and Grasser, Marion and Nielsen, Kare L. and Grasser, Klaus D. (2011) Reduced expression of the DOG1 gene in Arabidopsis mutant seeds lacking the transcript elongation factor TFIIS. FEBS LETTERS, 585 (12). pp. 1929-1933. ISSN 1873-3468,
Full text not available from this repository. (Request a copy)Abstract
TFIIS is a transcript elongation factor that facilitates transcription by RNA polymerase II through blocks to elongation. Arabidopsis plants lacking TFIIS are affected in seed dormancy, which represents a block to complete germination under favourable conditions. We have comparatively profiled the transcript levels of seeds of tfIIs mutants and control plants. Among the differentially expressed genes, the DOG1 gene was identified that is a QTL for seed dormancy. The reduced expression of DOG1 in tfIIs seeds was confirmed by quantitative RT-PCR and Northern analyses, suggesting that down-regulation of DOG1 expression is involved in the seed dormancy phenotype of tfIIs mutants. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Item Type: | Article |
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Uncontrolled Keywords: | RNA-POLYMERASE-II; EXPERIMENTAL PROTOCOL; DORMANCY; SENSITIVITY; INITIATION; THALIANA; DEEPSAGE; LONGSAGE; SAMPLES; GROWTH; TFIIS; Transcript elongation; Seed dormancy; Transcript profiling |
Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
Depositing User: | Dr. Gernot Deinzer |
Date Deposited: | 09 Jun 2020 06:34 |
Last Modified: | 09 Jun 2020 06:34 |
URI: | https://pred.uni-regensburg.de/id/eprint/20650 |
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