Chambers, Karen F. and Pearson, Joanna F. and Pellacani, Davide and Aziz, Naveed and Guzvic, Miodrag and Klein, Christoph A. and Lang, Shona H. (2011) Stromal upregulation of lateral epithelial adhesions: Gene expression analysis of signalling pathways in prostate epithelium. JOURNAL OF BIOMEDICAL SCIENCE, 18: 45. ISSN 1021-7770,
Full text not available from this repository. (Request a copy)Abstract
Background: Stromal signalling increases the lateral cell adhesions of prostate epithelial cells grown in 3D culture. The aim of this study was to use microarray analysis to identify significant epithelial signalling pathways and genes in this process. Methods: Microarray analysis was used to identify genes that were differentially expressed when epithelial cells were grown in 3D Matrigel culture with stromal co-culture compared to without stroma. Two culture models were employed: primary epithelial cells (ten samples) and an epithelial cell line (three experiments). A separate microarray analysis was performed on each model system and then compared to identify tissue-relevant genes in a cell line model. Results: TGF beta signalling was significantly ranked for both model systems and in both models the TGF beta signalling gene SOX4 was significantly down regulated. Analysis of all differentially expressed genes to identify genes that were common to both models found several morphology related gene clusters; actin binding (DIAPH2, FHOD3, ABLIM1, TMOD4, MYH10), GTPase activator activity (BCR, MYH10), cytoskeleton (MAP2, MYH10, TMOD4, FHOD3), protein binding (ITGA6, CD44), proteinaceous extracellular matrix (NID2, CILP2), ion channel/ion transporter activity (CACNA1C, CACNB2, KCNH2, SLC8A1, SLC39A9) and genes associated with developmental pathways (POFUT1, FZD2, HOXA5, IRX2, FGF11, SOX4, SMARCC1). Conclusions: In 3D prostate cultures, stromal cells increase lateral epithelial cell adhesions. We show that this morphological effect is associated with gene expression changes to TGF beta signalling, cytoskeleton and anion activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR-BETA; CELL-ADHESION; OLIGONUCLEOTIDE ARRAYS; E-CADHERIN; PROTEIN; ACTIN; CANCER; MORPHOGENESIS; JUNCTIONS; MECHANISM; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jun 2020 08:35 |
| Last Modified: | 09 Jun 2020 08:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20651 |
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