Becker, Marc and Reuter, Sebastian and Friedrich, Pamela and Doener, Fatma and Michel, Anastasija and Bopp, Tobias and Klein, Matthias and Schmitt, Edgar and Schild, Hansjoerg and Radsak, Markus P. and Echtenacher, Bernd and Taube, Christian and Stassen, Michael (2011) Genetic Variation Determines Mast Cell Functions in Experimental Asthma. JOURNAL OF IMMUNOLOGY, 186 (12). pp. 7225-7231. ISSN 0022-1767,
Full text not available from this repository. (Request a copy)Abstract
Mast cell-deficient mice are a key for investigating the function of mast cells in health and disease. Allergic airway disease induced as a Th2-type immune response in mice is employed as a model to unravel the mechanisms underlying inception and progression of human allergic asthma. Previous work done in mast cell-deficient mouse strains that otherwise typically mount Th1-dominated immune responses revealed contradictory results as to whether mast cells contribute to the development of airway hyperresponsiveness and airway inflammation. However, a major contribution of mast cells was shown using adjuvant-free protocols to achieve sensitization. The identification of a traceable genetic polymorphism closely linked to the Kit(W-sh) allele allowed us to generate congenic mast cell-deficient mice on a Th2-prone BALB/c background, termed C. B6-Kit(W-sh). In accordance with the expectations, C. B6-Kit(W-sh) mice do not develop IgE-and mast cell-dependent passive cutaneous anaphylaxis. Yet, unexpectedly, C. B6-Kit(W-sh) mice develop full-blown airway inflammation, airway hyperresponsiveness, and mucus production despite the absence of mast cells. Thus, our findings demonstrate a major influence of genetic background on the contribution of mast cells in an important disease model and introduce a novel strain of mast cell-deficient mice. The Journal of Immunology, 2011, 186: 7225-7231.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ALLERGIC AIRWAY INFLAMMATION; C-KIT EXPRESSION; FC-EPSILON-RI; W-SH; MICE; DEFICIENT; RESPONSES; LOCUS; MODEL; MOUSE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jun 2020 09:02 |
| Last Modified: | 09 Jun 2020 09:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20660 |
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