Amusquivar, Encarnacion and Schiffner, Susanne and Herrera, Emilio (2011) Evaluation of two methods for plasma fatty acid analysis by GC. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 113 (6). pp. 711-716. ISSN 1438-7697,
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The present study was designed to establish the best method for the quantitative analysis of fatty acids in blood plasma. In the "two steps" method, internal standards of C15:0, C19:0, C19:1, or C21:0 fatty acids were added to plasma and lipids were extracted in chloroform/methanol (2:1). Lipid extracts resuspended in methanol/toluene (4:1) were subjected to methanolysis at 80 degrees C for 2.5 h with acetyl chloride for GC analysis. In the "direct" method, plasma containing internal standards were suspended in methanol/toluene and after methanolysis at 100 degrees C for 1 h with acetyl chloride were processed as above. When C15:0 was used to calculate recoveries, values were above 100%, these being higher when using the "direct" method. When methylated C17:0 was the reference standard, the peak area ratios between internal standards and methylated reference were lower for C15:0 than for any of the others. When using C19:0 as the internal standard, quantitative values of individual fatty acids were higher when the "two steps" method was used as compared to the "direct" method. Thus, the "two steps" method is more appropriate and reliable for the quantitative analysis of fatty acid profiles in plasma samples, and C19:0 but not C15:0 should be used as the internal standard.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GAS-CHROMATOGRAPHY; QUANTIFICATION; METHYLATION; Internal standards; Plasma; Quantitative fatty acid analysis; Recovery |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jun 2020 10:45 |
| Last Modified: | 09 Jun 2020 10:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20705 |
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