Eberhart, Karin and Rainer, Johannes and Bindreither, Daniel and Ritter, Ireen and Gnaiger, Erich and Kofler, Reinhard and Oefner, Peter J. and Renner, Kathrin (2011) Glucocorticoid-induced alterations in mitochondrial membrane properties and respiration in childhood acute lymphoblastic leukemia. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1807 (6). pp. 719-725. ISSN 0005-2728,
Full text not available from this repository. (Request a copy)Abstract
Mitochondria are signal-integrating organelles involved in cell death induction. Mitochondrial alterations and reduction in energy metabolism have been previously reported in the context of glucocorticoid (Cc)triggered apoptosis, although the mechanism is not yet clarified. We analyzed mitochondrial function in a GC-sensitive precursor B-cell acute lymphoblastic leukemia (ALL) model as well as in GC-sensitive and GC-resistant T-ALL model systems. Respiratory activity was preserved in intact GC-sensitive cells up to 24 h under treatment with 100 nM dexamethasone before depression of mitochondrial respiration occurred. Severe repression of mitochondrial respiratory function was observed after permeabilization of the cell membrane and provision of exogenous substrates. Several mitochondrial metabolite and protein transporters and two subunits of the ATP synthase were downregulated in the T-ALL and in the precursor B-ALL model at the gene expression level under dexamethasone treatment. These data could partly be confirmed in ALL lymphoblasts from patients, dependent on the molecular abnormality in the ALL cells. GC-resistant cell lines did not show any of these defects after dexamethasone treatment. In conclusion, in GC-sensitive ALL cells, dexamethasone induces changes in membrane properties that together with the reduced expression of mitochondrial transporters of substrates and proteins may lead to repressed mitochondrial respiratory activity and lower ATP levels that contribute to GC-induced apoptosis. This article is part of a Special Issue entitled: Bioenergetics of Cancer. (C) 2010 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYTOCHROME-C RELEASE; INDUCED APOPTOSIS; ENERGY-METABOLISM; CELLS; RESISTANCE; OVEREXPRESSION; CATALASE; CARRIERS; RECEPTOR; DEFECTS; Glucocorticoid; Acute lymphoblastic leukemia; Mitochondrial transport; Mitochondrial membrane properties; Mitochondrial respiration; Apoptosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jun 2020 12:08 |
| Last Modified: | 09 Jun 2020 12:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20724 |
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