Bredl, Simon and Plentz, Annelie and Wenzel, Juergen J. and Pfister, Heiko and Moest, Johannes and Modrow, Susanne (2011) False-negative serology in patients with acute parvovirus B19 infection. JOURNAL OF CLINICAL VIROLOGY, 51 (2). pp. 115-120. ISSN 1386-6532,
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Background: Acute parvovirus B19 (B19V) infection is characterized by high-level viremia. Antibodies against the capsid proteins VP1 and VP2 may complex with B19V-particles thereby becoming undetectable in diagnostic tests. Objectives: We intended to obtain data on the frequency of false-negative serology in acute B19V-infection. Study design: 129 plasma or serum samples of healthy blood donors and of patients with suspected B19V-infection were analyzed for B19V-DNA by qPCR and VP1/VP2-specific IgG and IgM by ELISA. Eleven of these samples were derived from four pregnant women with previous contact to B19V-infected individuals. Using acidic conditions virus/antibody-complexes were disrupted and detected by WesternLine and ELISA. Results: 83/118 samples were derived from acutely infected individuals displaying viremia (10(3)-10(12) geq/mL). In 24/83 viremic samples (28.9%) VP1/VP2-specific IgM and IgG were undetectable in ELISA, but could be demonstrated to be complexed with B19V-particles. Each 7/83 (8.4%) was IgM-positive/IgG-negative and IgM-negative/IgG-positive, in 45/83 samples (54.2%) IgG and IgM could be detected. 35 samples did not contain B19V-DNA; five of these were from seronegative persons. Analyzing consecutive sera derived from four pregnant women, B19V-DNA was demonstrated in 10/11 samples, B19V-specific IgG- and IgM-antibodies were detectable in 10/11 and 4/11 samples, respectively. In 2/4 women seroconversion was observed, but IgM was not detected in 50% of the samples. B19V-specific IgG but not IgM was detectable in 2/4 women. Conclusion: Acute B19V-infection cannot be diagnosed by exclusive analysis of B19V-specific antibodies. Only the combination of assays for detection of B19V-DNA and antibodies enables correct serodiagnosis. (C) 2011 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VP2 LINEAR EPITOPES; PREGNANCY; HEPATITIS; RESPONSES; VIREMIA; ANTIBODIES; ANTIGEN; VIRUS; TIME; CLEARANCE; Parvovirus B19; Acute infection; Immunocomplex formation; Viremia; Pregnancy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Jun 2020 12:57 |
| Last Modified: | 10 Jun 2020 12:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20726 |
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