Mauerer, Andreas and Roesch, Alexander and Hafner, Christian and Stempfl, Thomas and Wild, Peter and Meyer, Stefanie and Landthaler, Michael and Vogt, Thomas (2011) Identification of new genes associated with melanoma. EXPERIMENTAL DERMATOLOGY, 20 (6). pp. 502-507. ISSN 0906-6705, 1600-0625
Full text not available from this repository. (Request a copy)Abstract
Purpose: Repeated failures in melanoma therapy made clear that the molecular mechanisms leading to melanoma are still poorly understood. In this study, we aim to provide a more comprehensive understanding of the transcriptional profiles and signalling pathways associated with melanoma. Methods: Gene expression was analysed using the Affymetrix Human Genome U133A 2.0 GeneChip arrays. To avoid culture artifacts, we used microdissected fresh frozen material of 18 melanocytic nevi (MN), 20 primary melanomas (PM) and 20 metastatic melanomas (MM). Statistical analysis was performed with Genomatix Chipinspector, Ingenuity (TM) Software, SPSS Software and Partek Genomic Suite 6.4. Expression levels of selected transcripts were verified by quantitative real-time RT-PCR and immunostaining of a tissue microarray sampling more than 280 cases of MN, PM and MM with known clinical outcome. Results: A total of 284 differentially expressed genes was detected in PM compared with MN and 189 genes in MM compared with PM affecting common cancer pathways such as MAPK-, Wnt- and Notch-signalling. Using principal component analysis, the samples could be grouped according to their histological entity. We identified a panel of novel melanoma-associated markers: frizzled-related protein, an antagonist of Wnt; tranducin-like enhancer of split 1, a transcription factor partner of TCF/LEF-1; CNTN1, an activator of Notch signalling; two Serpin peptidase inhibitors, Serpin B3/B4 and the TGF-beta family member GDF15, the latter with association to MAPK-signalling.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MACROPHAGE INHIBITORY CYTOKINE-1; SQUAMOUS-CELL CARCINOMA; E-CADHERIN EXPRESSION; MALIGNANT-MELANOMA; METASTATIC MELANOMA; MELANOCYTIC LESIONS; TISSUE MICROARRAYS; TUMOR PROGRESSION; HIGH-THROUGHPUT; HUMAN CANCER; FRZB; melanoma; Serpin B3; Serpin B4; TLE1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jun 2020 09:53 |
| Last Modified: | 15 Jun 2020 09:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20745 |
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