Klein, Matthias S. and Dorn, Christoph and Saugspier, Michael and Hellerbrand, Claus and Oefner, Peter J. and Gronwald, Wolfram (2011) Discrimination of steatosis and NASH in mice using nuclear magnetic resonance spectroscopy. METABOLOMICS, 7 (2). pp. 237-246. ISSN 1573-3882, 1573-3890
Full text not available from this repository. (Request a copy)Abstract
Nonalcoholic fatty liver disease (NAFLD) is a common cause of hepatic dysfunction. The disease spectrum ranges from hepatic steatosis to nonalcoholic steatohepatitis (NASH). The aim of this study was to identify metabolic differences in murine models of simple hepatic steatosis and NASH for the distinction of these NAFLD stages. For 12 weeks, male BALB/c mice were fed either a control or two different high-fat diets leading to hepatic steatosis and NASH, respectively. Metabolic differences were determined by independent component analysis (ICA) of nuclear magnetic resonance (NMR) spectra of lipophilic and hydrophilic liver extracts, and urine specimens. The results from ICA clearly discriminated the three investigated groups. Discriminatory biomarkers in the lipophilic liver extracts were free cholesterol, cholesterol ester and lipid methylene. Discrimination of the hydrophilic liver extracts was mainly mediated by betaine, glucose, and lactate, whereas in urine taurine, trimethylamine-N-oxide, and trimethylamine were the most discriminatory biomarkers. In conclusion, NMR metabolite fingerprinting of spot urine specimens may allow the noninvasive distinction of steatosis and NASH.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FATTY LIVER-DISEASE; INDEPENDENT COMPONENT ANALYSIS; NONALCOHOLIC STEATOHEPATITIS; RATS; NMR; TAURINE; OBESITY; URINE; Nonalcoholic fatty liver disease; Metabolomics; Urine; NMR |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jun 2020 10:00 |
| Last Modified: | 15 Jun 2020 10:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20749 |
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