Estelmann, Sebastian and Ramos-Vera, Walter Hugo and Gad'on, Nasser and Huber, Harald and Berg, Ivan A. and Fuchs, Georg (2011) Carbon dioxide fixation in 'Archaeoglobus lithotrophicus': are there multiple autotrophic pathways? FEMS MICROBIOLOGY LETTERS, 319 (1). pp. 65-72. ISSN 0378-1097,
Full text not available from this repository. (Request a copy)Abstract
Several representatives of the euryarchaeal class Archaeoglobi are able to grow facultative autotrophically using the reductive acetyl-CoA pathway, with 'Archaeoglobus lithotrophicus' being an obligate autotroph. However, genome sequencing revealed that some species harbor genes for key enzymes of other autotrophic pathways, i.e. 4-hydroxybutyryl-CoA dehydratase of the dicarboxylate/hydroxybutyrate cycle and the hydroxypropionate/hydroxybutyrate cycle and ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) of the Calvin-Benson cycle. This raised the question of whether only one or multiple autotrophic pathways are operating in these species. We searched for the presence of enzyme activities specific for the dicarboxylate/hydroxybutyrate or the hydroxypropionate/hydroxybutyrate cycles in 'A. lithotrophicus', but such enzymes could not be detected. Low Rubisco activity was detected that could not account for the carbon dioxide (CO(2)) fixation rate; in addition, phosphoribulokinase activity was not found. The generation of ribulose 1,5-bisphosphate from 5-phospho-d-ribose 1-pyrophosphate was observed, but not from AMP; these sources for ribulose 1,5-bisphosphate have been proposed before. Our data indicate that the reductive acetyl-CoA pathway is the only functioning CO(2) fixation pathway in 'A. lithotrophicus'.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SP-NOV.; CO2 FIXATION; 3-HYDROXYPROPIONATE CYCLE; RIBULOSE 1,5-BISPHOSPHATE; ESCHERICHIA-COLI; SULFATE-REDUCER; COENZYME-A; GEN. NOV.; ARCHAEA; FULGIDUS; Archaeoglobus lithotrophicus; 4-hydroxybutyryl-CoA dehydratase; autotrophy; dicarboxylate; hydroxybutyrate cycle; hydroxypropionate; hydroxybutyrate cycle; ribulose 1; 5-bisphosphate carboxylase |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jun 2020 07:34 |
| Last Modified: | 15 Jun 2020 07:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20792 |
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