Oelkers, J. Margit and Vinzenz, Marlene and Nemethova, Maria and Jacob, Sonja and Lai, Frank P. L. and Block, Jennifer and Szczodrak, Malgorzata and Kerkhoff, Eugen and Backert, Steffen and Schlueter, Kai and Stradal, Theresia E. B. and Small, J. Victor and Koestler, Stefan A. and Rottner, Klemens (2011) Microtubules as Platforms for Assaying Actin Polymerization In Vivo. PLOS ONE, 6 (5): e19931. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
The actin cytoskeleton is continuously remodeled through cycles of actin filament assembly and disassembly. Filaments are born through nucleation and shaped into supramolecular structures with various essential functions. These range from contractile and protrusive assemblies in muscle and non-muscle cells to actin filament comets propelling vesicles or pathogens through the cytosol. Although nucleation has been extensively studied using purified proteins in vitro, dissection of the process in cells is complicated by the abundance and molecular complexity of actin filament arrays. We here describe the ectopic nucleation of actin filaments on the surface of microtubules, free of endogenous actin and interfering membrane or lipid. All major mechanisms of actin filament nucleation were recapitulated, including filament assembly induced by Arp2/3 complex, formin and Spir. This novel approach allows systematic dissection of actin nucleation in the cytosol of live cells, its genetic re-engineering as well as screening for new modifiers of the process.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECEPTOR-MEDIATED ENDOCYTOSIS; ALDRICH-SYNDROME PROTEIN; ARP2/3 COMPLEX; N-WASP; FILOPODIA FORMATION; WASP/SCAR PROTEINS; NUCLEATION FACTOR; CELL-ADHESION; LEADING-EDGE; CORDON-BLEU; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jun 2020 09:09 |
| Last Modified: | 15 Jun 2020 09:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20820 |
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