Wittner, Manuel and Hamperl, Stephan and Stoeckl, Ulrike and Seufert, Wolfgang and Tschochner, Herbert and Milkereit, Philipp and Griesenbeck, Joachim (2011) Establishment and Maintenance of Alternative Chromatin States at a Multicopy Gene Locus. CELL, 145 (4). pp. 543-554. ISSN 0092-8674,
Full text not available from this repository. (Request a copy)Abstract
In eukaryotes, each of the more than 100 copies of ribosomal RNA (rRNA) genes exists in either an RNA polymerase I transcribed open chromatin state or a nucleosomal, closed chromatin state. Open rRNA genes guarantee the cell's supply with structural components of the ribosome, whereas closed rRNA genes ensure genomic integrity. We report that the observed balance between open and closed rRNA gene chromatin states in proliferating yeast cells is due to a dynamic equilibrium of transcription-dependent removal and replication-dependent assembly of nucleosomes. Pol I transcription is required for the association of the HMG box protein Hmo1 with open rRNA genes, counteracting replication-independent nucleosome deposition and maintaining the open rRNA gene chromatin state outside of S phase. The findings indicate that the opposing effects of replication and transcription lead to a de novo establishment of chromatin states for rRNA genes during each cell cycle.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RIBOSOMAL-RNA GENES; POLYMERASE-I TRANSCRIPTION; SACCHAROMYCES-CEREVISIAE; CELL-CYCLE; ACTIVE GENES; PROTEIN HMO1; BOX PROTEIN; YEAST; RDNA; UBF; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Philipp Milkereit Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Cell Cycle Control > Prof. Dr. Wolfgang Seufert |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jun 2020 14:39 |
| Last Modified: | 15 Jun 2020 14:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20826 |
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