Kritzenberger, Michaela and Junglas, Benjamin and Framme, Carsten and Helbig, Horst and Gabel, Veit-Peter and Fuchshofer, Rudolf and Tamm, Ernst R. and Hillenkamp, Jost (2011) Different collagen types define two types of idiopathic epiretinal membranes. HISTOPATHOLOGY, 58 (6). pp. 953-965. ISSN 0309-0167,
Full text not available from this repository. (Request a copy)Abstract
Different collagen types define two types of idiopathic epiretinal membranes Aims: To identify differences in extracellular matrix contents between idiopathic epiretinal membranes (IEM) of cellophane macular reflex (CMRM) or preretinal macular fibrosis (PMFM) type. Methods and results: Idiopathic epiretinal membranes were analysed by light and quantitative transmission electron microscopy, immunohistochemistry and Western blotting. Substantial differences between CMRM and PMFM were observed regarding the nature of extracellular fibrils. In CMRM the fibrils were thin, with diameters between 6 and 15 nm. Between the fibrils, aggregates of long-spacing collagen were observed. In PMFM the diameters of fibrils measured either 18-26 or 36-56 nm. Using immunogold electron microscopy, 6-15 nm fibrils in CMRM were labelled for collagen type VI, while the fibrils in PMFM remained unstained. Using Western blotting and immunohistochemistry, a strong signal for collagen type VI was observed in all CMRM, while immunoreactivity was weak or absent in PMFM. In contrast, PMFM showed immunoreactivity for collagen types I and II, which was weak or absent in CMRM. Both types of membranes showed immunoreactivity for collagen types III and IV, laminin and fibronectin with similar intensity. Conclusion: The presence of high amounts of collagen type VI in CMRM and the relative absence of collagen types I and II is the major structural difference to PMFM.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SMOOTH-MUSCLE-ACTIN; PROLIFERATIVE DIABETIC-RETINOPATHY; EXTRACELLULAR-MATRIX COMPONENTS; PRERETINAL MACULAR FIBROSIS; PIGMENT EPITHELIAL-CELLS; BLUE MOUNTAINS EYE; VI-COLLAGEN; MULLER CELLS; ULTRASTRUCTURAL FEATURES; VITREORETINAL INTERFACE; collagen type III; collagen type VI; epiretinal membrane; immunogold techniques; immunohistochemistry; transmission electron microscopy; Western blotting |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie > Prof. Dr. Ernst Tamm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Jun 2020 05:30 |
| Last Modified: | 18 Jun 2020 05:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20859 |
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