Felthaus, O. and Ettl, T. and Gosau, M. and Driemel, O. and Brockhoff, G. and Reck, A. and Zeitler, K. and Hautmann, M. and Reichert, T. E. and Schmalz, G. and Morsczeck, C. (2011) Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 407 (1). pp. 28-33. ISSN 0006-291X, 1090-2104
Full text not available from this repository. (Request a copy)Abstract
Resistance of oral squamous cell carcinomas (OSCC) to conventional chemotherapy or radiation therapy might be due to cancer stem cells (CSCs). The development of novel anticancer drugs requires a simple method for the enrichment of CSCs. CSCs can be enriched from OSCC cell lines, for example, after cultivation in serum-free cell culture medium (SFM). In our study, we analyzed four OSCC cell lines for the presence of CSCs. CSC-like cells could not be enriched with SFM. However, cell lines obtained from holoclone colonies showed CSC-like properties such as a reduced rate of cell proliferation and a reduced sensitivity to Paclitaxel in comparison to cells from the parental lineage. Moreover, these cell lines differentially expressed the CSC-marker CD133, which is also upregulated in OSCC tissues. Interestingly, CD133(+) cells in OSCC tissues expressed little to no Ki67, the cell proliferation marker that also indicates reduced drug sensitivity. Our study shows a method for the isolation of CSC-like cell lines from OSCC cell lines. These CSC-like cell lines could be new targets for the development of anticancer drugs under in vitro conditions. (C) 2011 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INITIATING CELLS; LUNG-CANCER; NECK-CANCER; HEAD; BIOLOGY; OCT-4; A549; Cancer stem cells; Oral squamous cell carcinoma; WNT signaling; Colony forming; Cell line |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Pathologie Medicine > Lehrstuhl für Strahlentherapie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jun 2020 05:31 |
| Last Modified: | 23 Jun 2020 05:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21031 |
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