Kumar, Ashwini and Burns, Jacob and Hoffmann, Werner and Demattio, Horst and Malik, Ashok Kumar and Matysik, Frank-Michael (2011) Determination of hydrazines by chip electrophoresis with contactless conductivity detection. ELECTROPHORESIS, 32 (8). pp. 920-925. ISSN 0173-0835,
Full text not available from this repository. (Request a copy)Abstract
In this report, a new approach for the fast determination of hydrazine compounds (hy) in complex matrices is presented. The experimental protocol is based on poly(methylmethacrylate) (PMMA) microchip separations with contactless conductivity detection using a compact portable device, which integrates all separation and detection components. Three hy (hydrozine (Hy), methylhydrazine (MH), and 1,1-dimethylhydrazine (UDMH)) were separated within < 30 s at a separation voltage of 3.8 kV using a L(-)-histidine/2-(N-morpholinoethanesulfonic acid) (His/MES) buffer (25:50 mM, pH 5.87). The contactless conductivity detection enables detection limits for Hy, MH, and UDMH of 11.9, 35.5, and 337.8 ng/mL, respectively, with linear concentration dependence up to 10 mu g/mL. In complex matrices such as soil samples or river water, interferences were eliminated by implementing ultrasound-assisted headspace single-drop microextraction of hy under strongly alkaline conditions, using an aqueous drop of His/MES buffer as the extractant phase. The incorporation of this miniaturized sample preparation step led to improved limits of detection for Hy, MH, and UDMH of 6.5, 15.3, and 11.4 ng/mL, respectively. The overall protocol demonstrates a promising approach for interfacing chip electrophoresis with real-world applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ON-A-CHIP; MODIFIED ELECTROCHEMICAL DETECTOR; CAPILLARY-ELECTROPHORESIS; MICROCHIP ELECTROPHORESIS; METHYLHYDRAZINE; ELECTRODE; Chip electrophoresis; Contactless conductivity detection; Headspace single-drop microextraction; Hydrazines |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jun 2020 05:34 |
| Last Modified: | 23 Jun 2020 05:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21033 |
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