Grundmann, Marco and Matysik, Frank-Michael (2011) Fast capillary electrophoresis-time-of-flight mass spectrometry using capillaries with inner diameters ranging from 75 to 5 mu m. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 400 (1). pp. 269-278. ISSN 1618-2642, 1618-2650
Full text not available from this repository. (Request a copy)Abstract
Fast electrophoretic separations in fused silica capillaries (CE) coupled to time-of-flight mass spectrometry (TOF-MS) are presented. CE separations of the model analytes (epinephrine, norepinephrine, dopamine, histidine, and isoproterenol) under conditions of high electric field strengths of up to 1.25 kV cm(-1) are completed in 20 s. Coupling of CE with TOF-MS is accomplished using a coaxial sheath liquid electrospray ionization interface. The influence of parameters inherent to the interface and their effects, including suction pressure and dilution, are discussed. In addition to standard capillaries of 75 and 50 mu m inner diameter (ID), separations in capillaries with IDs of 25, 15, and 5 mu m have been successfully applied to this setup. The analytical performance is compared over this range of capillary dimensions, and both advantages and disadvantages are discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPREHENSIVE 2-DIMENSIONAL SYSTEM; ELECTRICAL-FIELD STRENGTHS; FUSED-SILICA CAPILLARIES; FLOW GATING INTERFACE; ELECTROSPRAY-IONIZATION; ZONE-ELECTROPHORESIS; ELECTROCHEMICAL DETECTION; FLUORESCENCE DETECTION; MICROCOLUMN-LC; SINGLE CELLS; Capillary electrophoresis; Time-of-flight mass spectrometry; Electrospray ionization; Fused silica capillaries; High electric field strengths; Catecholamines |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jun 2020 07:11 |
| Last Modified: | 23 Jun 2020 07:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21063 |
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