Shaikhibrahim, Zaki and Langer, Berit and Lindstrot, Andreas and Florin, Alexandra and Bosserhoff, Anja and Buettner, Reinhard and Wernert, Nicolas (2011) Ets-1 is implicated in the regulation of androgen co-regulator FHL2 and reveals specificity for migration, but not invasion, of PC3 prostate cancer cells. ONCOLOGY REPORTS, 25 (4). pp. 1125-1129. ISSN 1021-335X, 1791-2431
Full text not available from this repository. (Request a copy)Abstract
Different members of the Ets-family of transcription factors are involved in TMPRSS-2-Ets translocations frequently found in human prostate cancers. We previously reported that Ets-1, which is the prototype of Ets-family members, promotes both migration and invasion of melanoma, HeLa and glioma cells. Here, we examined whether Ets-1 has a similar effect upon migration and invasion of PC3 prostate cancer cells, and whether it is implicated in the regulation of the androgen co-regulator four and a half LIM only protein-2 (FHL2). Two stable PC3 cell cultures were established by transfection with either an Ets-1 inverse antisense expression vector or a mock control vector. Western blot analysis confirmed presence of Ets-1 in mock and absence in Ets-1 inverse cells. Microarray and qRT-PCR revealed an up-regulation of FHL2 in Ets-1 blocked cells, compared to mock. To examine the effects of Ets-1 upon cell migration, a wound assay was performed, and demonstrated that wounds were completely colonized by mock compared to Ets-1 blocked cells after 55 h. Evaluation of the effect upon invasion was examined using the Boyden chamber, which revealed no significant difference between mock and Ets-1 blocked cells. In conclusion, our study demonstrated for the first time that Ets-1 is implicated in the regulation of the androgen co-regulator FHL2, and reveals specificity of action for migration, but not invasion of PC3 prostate cancer cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSCRIPTION FACTOR ETS-1; STROMAL EXPRESSION; BREAST-CANCER; TUMOR-CELLS; IN-VIVO; METASTASIS; GENE; ANGIOGENESIS; COACTIVATOR; RECEPTOR; PC3 prostate cancer cells; migration; invasion; Ets-1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jun 2020 07:15 |
| Last Modified: | 23 Jun 2020 07:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21066 |
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