Altman, Andrew M. and Prantl, Lukas and Muehlberg, Fabian L. and Song, Yao-Hua and Seidensticker, Max and Butler, Charles E. and Alt, Eckhard U. (2011) Wound Microenvironment Sequesters Adipose-Derived Stem Cells in a Murine Model of Reconstructive Surgery in the Setting of Concurrent Distant Malignancy. PLASTIC AND RECONSTRUCTIVE SURGERY, 127 (4). pp. 1467-1477. ISSN 0032-1052,
Full text not available from this repository. (Request a copy)Abstract
Background: It is unclear whether mesenchymal stem cells that are applied to regenerate wound tissues can migrate to existing tumors and enhance their growth. The authors investigated whether adipose-derived stern cells had any effect on the growth and progression of distant tumors when applied to a skin wound. Methods: The authors subcutaneously injected murine 4T1 breast cancer cells into all BALB/c-nu/nu mice. After tumor injection, mice were randomized to five groups (five mice per group) based on the means of co-introduction of green fluorescent protein labeled adipose-derived stem cells, if any. In group I, adipose-derived stem cells were combined and co-injected subcutaneously. In group 2, they were injected subcutaneously at a distant anatomical site. In group 3, they were injected intravenously. In group 4, they were delivered via a human acellular dermal matrix to a distant skin wound. In group 5, no adipose-derived stem cells were introduced. Results: After 2 weeks, tumor volume increased in group 1 (356.5 +/- 44.4 mm(3)), followed by group 3 (256.6 +/- 47.1 mm(3)) and then group 2 (201.6 +/- 28.6 mm(3)). In group 4, in which adipose-derived stem cells carried on acellular dermal matrix were applied to a wound distant to the primary tumor, the tumor volume was 143.8 +/- 50.9 mm(3), which was similar to that observed in the control group (group 5; 167.8 +/- 29.9 mm(3)). Conclusions: The authors' findings suggest that the wound microenvironment can retain adipose-derived stem cells, preventing their homing and stromal contribution to a distant neoplastic focus. These findings are an important first step in establishing the feasibility and safety of utilizing adipose-derived stem cell therapy for reconstructive surgery in the setting of malignancy. (Plast. Reconstr. Surg. 127: 1467, 2011.)
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SENTINEL LYMPH-NODE; STROMAL CELLS; BONE-MARROW; IN-VIVO; MYOCARDIAL-INFARCTION; AUTOCRINE GROWTH; HEPATIC LINEAGE; CANCER-CELLS; TUMOR-GROWTH; TISSUE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jun 2020 07:17 |
| Last Modified: | 23 Jun 2020 07:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21067 |
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