Ratzinger, Sabine and Graessel, Susanne and Dowejko, Albert and Reichert, Torsten E. and Bauer, Richard J. (2011) Induction of type XVI collagen expression facilitates proliferation of oral cancer cells. MATRIX BIOLOGY, 30 (2). pp. 118-125. ISSN 0945-053X, 1569-1802
Full text not available from this repository. (Request a copy)Abstract
Type XVI collagen belongs to the family of fibril-associated collagens with interrupted triple helices (FACIT). Recently, high affinity to integrin alpha1beta1 has been shown allowing cells expressing those integrins to attach and spread on recombinant type XVI collagen. Here, we show that type XVI collagen is overexpressed in dysplastic areas of mucosal epithelium from oral squamous cell carcinoma (OSCC) patients. Induction of its expression in OSCC cell lines (COLXVI cells) leads to an increased expression of Kindlin-1. Moreover, we demonstrate a significantly increased Kindlin-1/beta1-integrin interaction. Additionally, we detected a higher number of activated beta1-integrins in COLXVI cells and found a neo-expression of alpha1 integrin subunit on these cells. FACS analysis revealed a significantly higher amount of COLXVI cells in S-phase and G2/M-phase 6 h after synchronisation leading to a markedly higher proliferation activity. Blocking beta1-integrins with a specific antibody resulted in reduced proliferation of COLXVI cells. In summary, we demonstrate that overexpression of type XVI collagen in aberrant oral keratinocytes leads to Kindlin-1 induction, increased Kindlin-1/beta1-integrin interaction, integrin activation and subsequently to a proliferative cellular phenotype. (C) 2011 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | KERATINOCYTES; KINDLIN-1; ADHESION; LOCALIZATION; FIBROBLASTS; CARCINOMA; Cell-matrix adhesion; Cell cycle; Invasion; Oral oncology; Collagen XVI |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Jun 2020 09:03 |
| Last Modified: | 24 Jun 2020 09:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21173 |
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