Aldehni, Fadi and Tang, Tong and Madsen, Kirsten and Plattner, Michael and Schreiber, Andrea and Friis, Ulla G. and Hammond, H. Kirk and Han, Pyung Lim and Schweda, Frank (2011) Stimulation of Renin Secretion by Catecholamines Is Dependent on Adenylyl Cyclases 5 and 6. HYPERTENSION, 57 (3). 460-U232. ISSN 0194-911X, 1524-4563
Full text not available from this repository. (Request a copy)Abstract
The sympathetic nervous system stimulates renin release from juxtaglomerular cells via the beta-adrenoreceptor-cAMP pathway. Recent in vitro studies have suggested that the calcium-inhibited adenylyl cyclases (ACs) 5 and 6 possess key roles in the control of renin exocytosis. To investigate the relative contribution of AC5 and AC6 to the regulation of renin release in vivo we performed experiments using AC5 and AC6 knockout mice. Male AC5(-/-) mice exhibited normal plasma renin concentrations, renal renin synthesis (mRNA and renin content), urinary volume, and systolic blood pressure. In male AC6(-/-) mice, plasma renin concentration (AC6(-/-) : 732 +/- 119; AC6(+/+) : 436 +/- 78 ng of angiotensin I per hour*mL(-1); P<0.05), and renin synthesis were stimulated associated with an increased excretion of dilute urine (1.55-fold; P<0.05) and reduced blood pressure (-10.6 mm Hg; P<0.001). Stimulation of plasma renin concentration by a single injection of the beta-adrenoreceptor agonist isoproterenol (10 mg/kg IP) was significantly attenuated in AC5(-/-) (male: -20%; female: -33%) compared with wild-type mice in vivo. The mitigation of the plasma renin concentration response to isoproterenol was even more pronounced in AC6(-/-) (male: -63%; female: -50% versus AC6(+/+)). Similarly, the effects of isoproterenol, prostaglandin E2, and pituitary adenylyl cyclase-activating polypeptide on renin release from isolated perfused kidneys were attenuated to a higher extent in AC6(-/-) (-51% to -98% versus AC6(+/+)) than in AC5(-/-) (-31% to 46% versus AC5(+/+)). In conclusion, both AC5 and AC6 are involved in the stimulation of renin secretion in vivo, and AC6 is the dominant isoforms in this process. (Hypertension. 2011;57:460-468.). Online Data Supplement
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | JUXTAGLOMERULAR CELLS; DIFFERENTIAL EXPRESSION; MICE DEFICIENT; CAMP CONTENT; RELEASE; INHIBITION; RECEPTORS; DELETION; DISRUPTION; ACTIVATION; ADCY5; ADCY6; renin; renal function; blood pressure |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Frank Schweda |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jun 2020 08:42 |
| Last Modified: | 25 Jun 2020 08:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21242 |
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