Meierhofer, Tanja and Rosnizeck, Ina C. and Graf, Thorsten and Reiss, Kerstin and Koenig, Burkhard and Kalbitzer, Hans Robert and Spoerner, Michael (2011) Cu(2+-)cyclen as Probe to Identify Conformational States in Guanine Nucleotide Binding Proteins. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133 (7). pp. 2048-2051. ISSN 0002-7863,
Full text not available from this repository. (Request a copy)Abstract
P-31 NMR spectroscopy is a suitable method for identifying conformational states in the active site of guanine nucleotide binding proteins detecting the nucleotide placed there. Because there is no labelinz necessary, this lmethod is gaining increasing interest. By (31P) NMR spectroscopy two major conformational states, namely state 1(T) and state 2(T), can be detected in active Ras protein characterized by different chemical shifts. Depending on the conformational state Ras shows clearly different physiological properties. Meanwhile analogous conformational equilibria could also be shown for other members of the Ras superfamily. It is often difficult to determine the conformational states of the proteins on the basis of chemical shift alone; therefore, direct detection would be a great advantage. With the use of Cu2+-cyclen which selectively interacts only with one of the major conformational states (state 1) one has a probe to distinguish between the two states, because only proteins existing in conformational state 1 interact with the Cu2+-cyclen at low millimolar concentrations. The suitability was proven using Ras(wt) and Ras mutants, Ras complexed with GTP, GppNHp, or GTP gamma S, as well as two further members of the Ras superfamily namely Arf1 and Ran.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RAS-BINDING; EFFECTOR INTERACTION; STRUCTURAL BASIS; GTP; EXCHANGE; COMPLEXES; DYNAMICS; DOMAIN; SWITCH; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jun 2020 09:50 |
| Last Modified: | 25 Jun 2020 09:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21254 |
Actions (login required)
 |
View Item |
