Schreml, Stephan and Meier, Robert J. and Wolfbeis, Otto S. and Landthaler, Michael and Szeimies, Rolf-Markus and Babilas, Philipp (2011) 2D luminescence imaging of pH in vivo. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108 (6). pp. 2432-2437. ISSN 0027-8424,
Full text not available from this repository. (Request a copy)Abstract
Luminescence imaging of biological parameters is an emerging field in biomedical sciences. Tools to study 2D pH distribution are needed to gain new insights into complex disease processes, such as wound healing and tumor metabolism. In recent years, luminescence-based methods for pH measurement have been developed. However, for in vivo applications, especially for studies on humans, biocompatibility and reliability under varying conditions have to be ensured. Here, we present a referenced luminescent sensor for 2D high-resolution imaging of pH in vivo. The ratiometric sensing scheme is based on time-domain luminescence imaging of FITC and ruthenium(II) tris-(4,7-diphenyl-1,10-phenanthroline). To create a biocompatible 2D sensor, these dyes were bound to or incorporated into microparticles (aminocellulose and polyacrylonitrile), and particles were immobilized in polyurethane hydrogel on transparent foils. We show sensor precision and validity by conducting in vitro and in vivo experiments, and we show the versatility in imaging pH during physiological and chronic cutaneous wound healing in humans. Implementation of this technique may open vistas in wound healing, tumor biology, and other biomedical fields.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLUORESCEIN ANGIOGRAPHY; MATHEMATICAL-MODEL; HYDROGEN-PEROXIDE; EXTRACELLULAR PH; STRATUM-CORNEUM; SKIN; GRADIENT; WOUNDS; OXYGEN; CA2+; hydrogen; sensors and probes; fluorescence; skin; dermatology |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jun 2020 05:18 |
| Last Modified: | 26 Jun 2020 05:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21275 |
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