Ruhl, Stefan and Berlenbach, Pereshia and Langenfelder, Sabine and Hoerl, Dagmar and Lehn, Norbert and Hiller, Karl-Anton and Schmalz, Gottfried and Durchschlag, Helmut (2011) Integrity of Proteins in Human Saliva after Sterilization by Gamma Irradiation. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77 (3). pp. 749-755. ISSN 0099-2240,
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Microbial contamination of whole human saliva is unwanted for certain in vitro applications, e. g., when utilizing it as a growth substratum for biofilm experiments. The aim of this investigation was to test gamma irradiation for its suitability to sterilize saliva and to investigate the treatment's influence on the composition and integrity of salivary proteins in comparison to filter sterilization. For inhibition of bacterial growth by gamma irradiation, a sterility assurance level of 10(-6) was determined to be reached at a dose of 3.5 kGy. At this dose, the integrity of proteins, as measured by fluorescence, circular dichroism, and gel electrophoretic banding pattern, and the enzymatic activities of salivary amylase and lysozyme were virtually unchanged. Filtration reduced the total protein concentration to about half of its original value and decreased lysozyme activity to about 10%. It can be concluded that irradiation is suitable for sterilizing whole saliva in its native form.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROLINE-RICH PROTEIN; STREPTOCOCCUS-MUTANS; ORAL ACTINOMYCES; IN-VITRO; LYSOZYME; GROWTH; IDENTIFICATION; BACTERIA; SURFACE; MUCUS; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jun 2020 07:22 |
| Last Modified: | 26 Jun 2020 07:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21303 |
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