Ziebart, Thomas and Walenta, Stefan and Kunkel, Martin and Reichert, Torsten E. and Wagner, Wilfried and Mueller-Klieser, Wolfgang (2011) Metabolic and proteomic differentials in head and neck squamous cell carcinomas and normal gingival tissue. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 137 (2). pp. 193-199. ISSN 0171-5216, 1432-1335
Full text not available from this repository. (Request a copy)Abstract
A high lactate content in malignant head and neck cancer (Head and neck squamous cell carcinomas, HNSCC) is associated with a higher risk of metastatic spread and lower overall patient survival. However, until present, the underlying mechanisms are not clearly understood. Here, a systematic comparison of glucose metabolism in HNSCC and homologous normal tissue is presented for the first time. The concentrations of glucose, lactate and ATP were measured in cryobiopsies of 29 human HNSCC and of 9 normal mucosa using bioluminescence imaging. The protein expression of lactate dehydrogenase (LDH) was analyzed by Western blotting. Tumors own a higher content of lactate and LDH in comparison with normal tissues. However, within the tumor group, the grade of LDH expression shows substantially strong variation and overlap with normal values. Furthermore, LDH expression was not correlated with tumor lactate content. Investigating a small subpopulation, patients with a short-term survival had significantly higher tumor lactate levels compared to patients with long-term survival. The data provide clear evidence of an enhanced glycolysis in tumors compared to normal tissue. This may partially but not completely attributable to an elevated expression of LDH. High tumor lactate levels may be predictive for restricted patient survival. In conclusion, lactate measurements, for example non-invasively with MRT, should be advanced for use in clinical routine as a supportive tool for tumor diagnosis and prognosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIGH LACTATE LEVELS; HYPOXIA-INDUCIBLE FACTOR-1; QUANTITATIVE BIOLUMINESCENCE; GLUCOSE-METABOLISM; AEROBIC GLYCOLYSIS; ATP CONCENTRATIONS; MALIGNANT-TUMORS; CANCER-CELLS; METASTASIS; OVEREXPRESSION; Head and neck squamous cell carcinomas; Normal tissue; Bioluminescence; Cancer metabolism; Metabolic imaging |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jun 2020 09:38 |
| Last Modified: | 26 Jun 2020 09:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21331 |
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