Spangler, B. and Vardimon, L. and Bosserhoff, A. K. and Kuphal, S. (2011) Post-transcriptional regulation controlled by E-cadherin is important for c-Jun activity in melanoma. PIGMENT CELL & MELANOMA RESEARCH, 24 (1). pp. 148-164. ISSN 1755-1471, 1755-148X
Full text not available from this repository. (Request a copy)Abstract
P>A central event in the development of malignant melanoma is the loss of the tumor-suppressor protein E-cadherin. Here, we report that this loss is linked to the activation of the proto-oncogene c-Jun, a key player in tumorigenesis. In vivo, malignant melanomas show strong expression of the c-Jun protein in contrast to melanocytes. Interestingly, c-Jun mRNA levels did not differ in the melanoma cell lines when compared to melanocytes, suggesting that c-Jun could be regulated at the post-transcriptional level. To uncover the link between E-cadherin and c-Jun, we re-expressed E-cadherin in melanoma cells and detected decreased protein expression and activity of c-Jun. Furthermore, c-Jun accumulation is dependent on active E-cadherin-mediated cell-cell adhesion and regulated via the cytoskeleton. Additionally, we determined that, with respect to c-Jun regulation, there are two melanoma subgroups. One subgroup regulates c-Jun expression via the newly discovered E-cadherin-dependent signaling pathway, whereas the other subgroup uses the MAPKinases to regulate its expression. In summary, our data provide novel insights into the tumor-suppressor function of E-cadherin, which contributes to the suppression of c-Jun protein translation and transcriptional activity independent of MAPKinases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | UVOMORULIN-CATENIN COMPLEX; MAMMALIAN UV RESPONSE; NF-KAPPA-B; MALIGNANT-MELANOMA; UP-REGULATION; TRANSCRIPTIONAL ACTIVITY; SKIN CARCINOGENESIS; SIGNALING PATHWAYS; CARCINOMA-CELLS; GASTRIC-CANCER; c-Jun; E-cadherin; malignant melanoma; post-transcriptional regulation; cytoskeleton |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jun 2020 09:42 |
| Last Modified: | 26 Jun 2020 09:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21333 |
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