Reiter, Alarich and Steinbauer, Robert and Philippi, Anja and Gerber, Jochen and Tschochner, Herbert and Milkereit, Philipp and Griesenbeck, Joachim (2011) Reduction in Ribosomal Protein Synthesis Is Sufficient To Explain Major Effects on Ribosome Production after Short-Term TOR Inactivation in Saccharomyces cerevisiae. MOLECULAR AND CELLULAR BIOLOGY, 31 (4). pp. 803-817. ISSN 0270-7306, 1098-5549
Full text not available from this repository. (Request a copy)Abstract
Ribosome synthesis depends on nutrient availability, sensed by the target of rapamycin (TOR) signaling pathway in eukaryotes. TOR inactivation affects ribosome biogenesis at the level of rRNA gene transcription, expression of ribosomal proteins (r-proteins) and biogenesis factors, preribosome processing, and transport. Here, we demonstrate that upon TOR inactivation, levels of newly synthesized ribosomal subunits drop drastically before the integrity of the RNA polymerase I apparatus is severely impaired but in good correlation with a sharp decrease in r-protein production. Inhibition of translation by cycloheximide mimics the rRNA maturation defect observed immediately after TOR inactivation. Both cycloheximide addition and the depletion of individual r-proteins also reproduce TOR-dependent nucleolar entrapment of specific ribosomal precursor complexes. We suggest that shortage of newly synthesized r-proteins after short-term TOR inactivation is sufficient to explain most of the observed effects on ribosome production.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-POLYMERASE-I; GENE-EXPRESSION; TRANSLATION INITIATION; CHROMATIN STRUCTURES; RAT-LIVER; SIGNALING PATHWAY; CELL-GROWTH; HELA-CELLS; YEAST; TRANSCRIPTION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Philipp Milkereit |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jun 2020 05:02 |
| Last Modified: | 29 Jun 2020 05:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21366 |
Actions (login required)
 |
View Item |
