Steiner, Mark-Steven and Duerkop, Axel (2011) Luminescent ruthenium probe for the determination of acetyl phosphate in complex biological matrices. ANALYST, 136 (1). pp. 148-154. ISSN 0003-2654, 1364-5528
Full text not available from this repository. (Request a copy)Abstract
The first probe for the fluorogenic determination of acetyl phosphate (AcP), (bpy)(2)Ru(1,10-phenanthroline- 5,6-dione dioxime) (RuPDO), was prepared and its reaction with AcP was studied in detail. The emission of the weakly luminescent RuPDO is red shifted and strongly enhanced upon reaction with AcP in the presence of metal cations like Zn2+ or Cu2+. The reaction occurs within 60 min incubation time under highly biocompatible conditions (aqueous buffer of pH 7, 37 degrees C). A linear dynamic range from 10 to 200 mu mol L-1 is observed with an LOD of AcP of 3.4 mu mol L-1 (for RuPDO-Zn). Other bio-phosphates studied show only weak interference. Furthermore, the applicability of the probe in complex biological matrices was evaluated.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACETATE KINASE; ESCHERICHIA-COLI; INORGANIC-PHOSPHATE; RESPONSE-REGULATOR; ACYL-PHOSPHATES; PATHWAY; ASSAY; PHOSPHORYLATION; HYDROLYSIS; SYSTEMS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jun 2020 06:49 |
| Last Modified: | 29 Jun 2020 06:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21461 |
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