Ulbrich, Claudia and Pietsch, Jessica and Grosse, Jirka and Wehland, Markus and Schulz, Herbert and Saar, Katrin and Huebner, Norbert and Hauslage, Jens and Hemmersbach, Ruth and Braun, Markus and van Loon, Jack and Vagt, Nicole and Egli, Marcel and Richter, Peter and Einspanier, Ralf and Sharbati, Soroush and Baltz, Theo and Infanger, Manfred and Ma, Xiao and Grimm, Daniela (2011) Differential Gene Regulation under Altered Gravity Conditions in Follicular Thyroid Cancer Cells: Relationship between the Extracellular Matrix and the Cytoskeleton. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 28 (2). pp. 185-198. ISSN 1015-8987,
Full text not available from this repository. (Request a copy)Abstract
Extracellular matrix proteins, adhesion molecules, and cytoskeletal proteins form a dynamic network interacting with signalling molecules as an adaptive response to altered gravity. An important issue is the exact differentiation between real microgravity responses of the cells or cellular reactions to hypergravity and/or vibrations. To determine the effects of real microgravity on human cells, we used four DLR parabolic flight campaigns and focused on the effects of short-term microgravity (22 s), hypergravity (1.8 g), and vibrations on ML-1 thyroid cancer cells. No signs of apoptosis or necrosis were detectable. Gene array analysis revealed 2430 significantly changed transcripts. After 22 s microgravity, the F-actin and cytokeratin cytoskeleton was altered, and ACTB and KRT80 mRNAs were significantly upregulated after the first and thirty-first parabolas. The COL4A5 mRNA was downregulated under microgravity, whereas OPN and FN were significantly upregulated. Hypergravity and vibrations did not change ACTB, KRT-80 or COL4A5 mRNA. MTSS1 and LIMA1 mRNAs were downregulated/slightly upregulated under microgravity, upregulated in hypergravity and unchanged by vibrations. These data indicate that the graviresponse of ML-1 cells occurred very early, within the first few seconds. Downregulated MTSS1 and upregulated LIMA1 may be an adaptive mechanism of human cells for stabilizing the cytoskeleton under microgravity conditions. Copyright (C) 2011 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL GROWTH-FACTOR; ROS 17/2.8 CELLS; SIMULATED MICROGRAVITY; INCREASES APOPTOSIS; MAMMALIAN-CELLS; ANGIOTENSIN-II; JURKAT CELLS; ADHESION; OSTEOPONTIN; WEIGHTLESSNESS; Thyroid cancer; Extracellular matrix; Apoptosis; Cytoskeleton; Weightlessness; Microgravity; Hypergravity; Vibration |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jun 2020 08:59 |
| Last Modified: | 29 Jun 2020 08:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21502 |
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