Bauer, Richard and Ratzinger, Sabine and Wales, Lynn and Bosserhoff, Anja and Senner, Volker and Grifka, Joachim and Graessel, Susanne (2011) Inhibition of Collagen XVI Expression Reduces Glioma Cell Invasiveness. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 27 (3-4). pp. 217-226. ISSN 1015-8987,
Full text not available from this repository. (Request a copy)Abstract
Glioblastomas are characterized by an intense local invasiveness that limits surgical resection. One mechanism by which glioma cells enforce their migration into brain tissue is reorganization of tumour associated extracellular matrix (ECM). Collagen XVI is a minor component of connective tissues. However, in glioblastoma tissue it is dramatically upregulated compared to the ECM of normal cortex. The aim of this study is to delineate tumour cell invasion and underlying mechanisms involving collagen XVI by using a siRNA mediated collagen XVI knockdown model in U87MG human glioblastoma cells. Knockdown of collagen XVI resulted in decreased invasiveness in Boyden chamber assays, and in a reduction of focal adhesion contact numbers per cell. Gene expression was upregulated for protocadherin 18 and downregulated for kindlin-1 and -2. Proliferation was not affected while flow cytometric analysis demonstrated reduced beta 1-integrin activation in collagen XVI knockdown cells. We suggest that in glioblastoma tissue collagen XVI may impair the cell-cell interaction in favour of enhancement of invasion. The modification of the beta 1-integrin activation pattern through collagen XVI might be a molecular mechanism to further augment the invasive phenotype of glioma cells. Elucidating the underlying mechanisms of glioma cell invasion promoted by collagen XVI may provide novel cancer therapeutic approaches in neurooncology. Copyright (C) 2011 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CANDIDATE TUMOR-SUPPRESSOR; EXTRACELLULAR-MATRIX; BRAIN; MIGRATION; INVASION; CANCER; PROTOCADHERINS; GLIOBLASTOMAS; FIBROBLASTS; INTEGRATION; Collagen XVI; Glioblastoma; Invasion; Protocadherin; Integrin; Kindlin; Activation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Orthopädie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jun 2020 12:52 |
| Last Modified: | 29 Jun 2020 12:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21507 |
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