Igl, Wilmar and Polasek, Ozren and Gornik, Olga and Knezevic, Ana and Pucic, Maja and Novokmet, Mislav and Huffman, Jennifer and Gnewuch, Carsten and Liebisch, Gerhard and Rudd, Pauline M. and Campbell, Harry and Wilson, James F. and Rudan, Igor and Gyllensten, Ulf and Schmitz, Gerd and Lauc, Gordan (2011) Glycomics meets lipidomics-associations of N-glycans with classical lipids, glycerophospholipids, and sphingolipids in three European populations. MOLECULAR BIOSYSTEMS, 7 (6). pp. 1852-1862. ISSN 1742-206X,
Full text not available from this repository. (Request a copy)Abstract
Recently, high-throughput technologies have been made available which allow the measurement of a broad spectrum of glycomics and lipidomics parameters in many samples. The aim of this study was to apply these methods and investigate associations between 46 glycan and 183 lipid traits measured in blood of 2041 Europeans from three different local populations (Croatia - VIS cohort; Sweden - NSPHS cohort; Great Britain - ORCADES cohort). N-glycans have been analyzed with High Performance Liquid Chromatography (HPLC) and lipids with Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS) covering sterol lipids, glycerolipids, glycerophospholipids and sphingolipids in eight subclasses. Overall, 8418 associations were calculated using linear mixed effect models adjusted for pedigree, sex, age and multiple testing. We found 330 significant correlations in VIS. Pearson's correlation coefficient r ranged from -0.27 to 0.34 with corresponding p-values between 1.45 x 10(-19) and 4.83 x 10(-6), indicating statistical significance. A total of 71 correlations in VIS could be replicated in NSPHS (r = [-0.19; 0.35], p = [4.16 x 10(-18); 9.38 x 10(-5)]) and 31 correlations in VIS were also found in ORCADES (r = [-0.20; 0.24], p = [2.69 x 10(-10); 7.55 x 10(-5)]). However, in total only 10 correlations between a subset of triantennary glycans and unsaturated phosphatidylcholine, saturated ceramide, and sphingomyelin lipids in VIS (r = [0.18; 0.34], p = [2.98 x 10(-21); 1.69 x 10(-06)]) could be replicated in both NSPHS and ORCADES. In summary, the results show strong and consistent associations between certain glycans and lipids in all populations, but also population-specific correlations which may be caused by environmental and genetic differences. These associations point towards potential interactive metabolic pathways.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; HIGH-THROUGHPUT QUANTIFICATION; MEMBRANE-LIPIDS; FATTY-ACIDS; ESI-MS/MS; DISEASE; GENOME; HEALTH; BIOLOGY; GLYCOSYLATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Jul 2020 06:26 |
| Last Modified: | 01 Jul 2020 06:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21625 |
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