Then, Cornelia and Bergler, Tobias and Jeblick, Roland and Jung, Bettina and Banas, Bernhard and Kraemer, Bernhard K. (2011) Hypertonic Stress Promotes the Upregulation and Phosphorylation of Zonula Occludens 1. NEPHRON PHYSIOLOGY, 119 (2). P11-P21. ISSN 1660-2137,
Full text not available from this repository. (Request a copy)Abstract
Tight junction molecules form a barrier between adjacent cells and mediate the cells' ability to develop membranes that constitute boundaries of different compartments within the body. Membranes with selective ion and water passage are important for the electrolyte and water homeostasis in the kidney. Due to their role in the urinary concentration process, renal medullary cells are exposed to hyperosmotic stress. Therefore, we were interested in the question of how mouse inner medullary collecting duct cells (mIMCD3) manage to maintain their cell-cell contacts, despite hypertonicity-induced cell shrinkage. Employing mRNA expression analysis, we found that the zonula occludens type 1 (Zo-1), multi-PDZ domain protein 1 (MUPP1) and cortactin mRNA levels were upregulated in a tonicity-dependent manner. Using Western blot analysis, immunoprecipitation and immunofluorescence, we show that the Zo-1 protein is upregulated, phosphorylated and linked to the actin cytoskeleton in response to hypertonic stress. After cell exposure to hypertonicity, rearrangement of the actin cytoskeleton resulted in a stronger colocalization of actin fibres with Zo-1. Urea, which generates hyperosmolality, but no transcellular gradient, did not induce changes in Zo-1 protein expression or actin rearrangement. This data indicates that Zo-1 is a response protein to inner medullary tonicity and that extracellular stressors can promote Zo-1 protein expression, tyrosine phosphorylation and cytoskeleton association. Copyright (C) 2011 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TIGHT JUNCTION PROTEIN; ACTIN CYTOSKELETON; OSMOTIC-STRESS; TYROSINE PHOSPHORYLATION; STIMULATES EXPRESSION; OSMOPROTECTIVE GENES; BARRIER FUNCTION; POTENTIAL ROLE; RAT-KIDNEY; MDCK CELLS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Jul 2020 08:29 |
| Last Modified: | 01 Jul 2020 08:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21633 |
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