Farwick, M. and Gauglitz, G. and Pavicic, T. and Koehler, T. and Wegmann, M. and Schwach-Abdellaoui, K. and Malle, B. and Tarabin, V. and Schmitz, G. and Korting, Hans Christian (2011) Fifty-kDa Hyaluronic Acid Upregulates Some Epidermal Genes without Changing TNF-alpha Expression in Reconstituted Epidermis. SKIN PHARMACOLOGY AND PHYSIOLOGY, 24 (4). pp. 210-217. ISSN 1660-5527, 1660-5535
Full text not available from this repository. (Request a copy)Abstract
Background: Due to its strong water binding potential, hyaluronic acid (HA) is a well-known active ingredient for cosmetic applications. However, based on its varying molecular size, skin penetration of HA may be limited. Recent studies have demonstrated that low-molecular-weight HA (LMW HA) may show a certain proinflammatory activity. We thus aimed to characterize an LMW-sized HA molecule that combines strong anti-aging abilities with efficient skin penetration but lacks potential proinflammatory effects. Methods: Total RNA and total protein were isolated from reconstituted human epidermis following incubation with HAs of various molecular weights (20, 50, 130, 300, 800 and 1,500 kDa). Tumor necrosis factor-alpha expression was determined using quantitative PCR. Genonnic and proteomic expression of various junctional proteins was determined using Affymetrix and common Western blotting techniques. Results: LMW HA of approximately 50 kDa did not significantly alter tumor necrosis factor-alpha expression compared to 20-kDa HA, but revealed significantly higher skin penetration rates than larger sized HA associated with increased expression of genes and proteins known to be involved in tight junction formation and keratinocyte cohesion. Conclusion: LMW HA of approximately 50 kDa shows better penetration abilities than larger-sized HA. In addition, LMW HA influences the expression of various genes including those contributing to keratinocyte differentiation and formation of intercellular tight junction complexes without showing proinflammatory activity. These observations contribute to current knowledge on the effects of LMW HA on keratinocyte biology and cutaneous physiology. Copyright (C) 2011 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TOPICALLY APPLIED SUBSTANCES; CD44 VARIANT ISOFORMS; HUMAN SKIN; STRATUM-CORNEUM; DENDRITIC CELLS; HAIR-FOLLICLES; FRAGMENTS; KERATINOCYTES; MACROPHAGES; TRANSCRIPTION; Hyaluronic acid; Skin penetration; Anti-wrinkle effect; Skin inflammation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jul 2020 05:42 |
| Last Modified: | 06 Jul 2020 05:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/21683 |
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