Kritzenberger, M. and Wrobel, K. -H. (2010) Role of the Mesonephros as a Transient Haematopoietic Organ in the Bovine Embryo. ANATOMIA HISTOLOGIA EMBRYOLOGIA, 39 (6). pp. 534-545. ISSN 0340-2096, 1439-0264
Full text not available from this repository. (Request a copy)Abstract
P>During vertebrate embryogenesis, haematopoietic stem cells (HSC) arise in the aorta-gonads-mesonephros (AGM) region. In the present study, we examined serial sections of 22-34 days post-insemination (dpi) bovine embryos, a species with a well-developed and functional mesonephros. We describe the temporo-spatial distribution of presumptive c-kit positive HSC and the occurrence of haematopoietic foci in the mesonephros and fetal liver using specific antibodies directed against haematopoietic cell markers and conventional electron microscopy. In the mesonephros, presumptive HSC were found at 23-24 dpi in the blood stream, in the endothelial lining of the filtering capillaries and in the septal stroma of the cranial part of the mesonephros, the mesonephric giant corpuscle (MGC), suggesting a colonalization via the blood stream from the haematopoietic clusters of the dorsal aorta. From 25 to 30 dpi, presumptive HSC predominate in the septal stroma of the MGC, were they first expand but then decline and disappear following 32 dpi. In parallel, we found ongoing erythropoiesis and myelopoiesis starting in the MGC at 24 dpi and extending during the complete observation period. In the embryonic liver, colonization with presumptive c-kit positive HSC occurs slightly later, at 25 dpi. Active formation of blood cells in the liver increases following 30 dpi. In conclusion, the mesonephros of bovine embryos, in particular its MGC, functions as a primitive haematopoietic organ, temporarily intercalated between extraembryonic erythropoiesis and haematopoiesis within in the fetal liver, thus recapitulating for a short period a phylogenetically old site of blood formation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PRIMORDIAL GERM-CELLS; STEM-CELLS; ELECTRON-MICROSCOPY; MOUSE EMBRYO; PROGENITOR CELLS; FETAL LIVER; INTRAEMBRYONIC HEMATOPOIESIS; STROMAL CELLS; YOLK-SAC; AORTA; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jul 2020 08:52 |
| Last Modified: | 06 Jul 2020 08:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23767 |
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