Schempp, Harald and Vogel, Susanne and Hueckelhoven, Ralph and Heilmann, Joerg (2010) Re-evaluation of superoxide scavenging capacity of xanthohumol. FREE RADICAL RESEARCH, 44 (12). pp. 1435-1444. ISSN 1071-5762,
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The chemopreventive chalcone xanthohumol (Xh) has been reported to decrease xanthine oxidase (XOD) catalysed formation of formazan from nitroblue tetrazolium (NBT) and is discussed as a potent scavenger of superoxide. Re-evaluation of the scavenging capacity indicated that Xh disturbed detection of superoxide with NBT, in case of an insufficient NBT/Xh ratio. Xh lacked superoxide scavenging activity in contrast to the Xh-derivative 3'-hydroxy-Xh with catechol substructure, used as positive control. This was shown by the use of sufficient concentration of NBT and other detectors such as hydroxylamine, XTT, cytochrome c and hydroethidine. HPLC analysis of reaction products in a xanthine/XOD/peroxidase system demonstrated beside enhanced inhibition of NBT-formazan by Xh that NBT even prevented oxidation of Xh. p-coumaric acid or ferulic acid could replace Xh in that system, indicating that superoxide detection using NBT is likely jeopardized by interference of phenoxyl-radicals. Furthermore, this study provides evidence that Xh can moderately generate superoxide via auto-oxidation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HABER-WEISS REACTION; HUMULUS-LUPULUS L.; ANTIOXIDATIVE ACTIVITY; NITROBLUE TETRAZOLIUM; PRENYLATED CHALCONES; SULFHYDRYL-GROUPS; XANTHINE-OXIDASE; XYLENOL ORANGE; ACID HYDRATE; REDUCTION; Superoxide; xanthohumol; 3 '-hydroxy-xanthohumol; bovine serum albumin; xanthine oxidase; NBT; XTT; cytochrome c; hydroxylamine; hydroethidine; horseradish peroxidase |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jul 2020 09:24 |
| Last Modified: | 06 Jul 2020 09:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23789 |
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