Roldan, J. C. and Chang, E. and Kelantan, M. and Jazayeri, L. and Deisinger, U. and Detsch, R. and Reichert, T. E. and Gurtner, G. C. (2010) Quantifying migration and polarization of murine mesenchymal stem cells on different bone substitutes by confocal laser scanning microscopy. JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY, 38 (8). pp. 580-588. ISSN 1010-5182, 1878-4119
Full text not available from this repository.Abstract
Introduction Cell migration is preceded by cell polarization The aim of the present study was to evaluate the impact of the geometry of different bone substitutes on cell morphology and chemical responses in vitro Materials and methods Cell polarization and migration were monitored temporally by using confocal laser scanning microscopy (CLSM) to follow green fluorescent protein (GFP) +/- mesenchymal stem cells (MSCs) on anorganic cancellous bovine bone (Bio-Oss (R)), beta-tricalcium phosphate (beta-TCP) (chronOS (R)) and highly porous calcium phosphate ceramics (Friedrich-Baur-Research-Institute for Biomaterials, Germany) Differentiation GFP +/- MSCs was observed using pro-angiogenic and pro-osteogenic biomarkers Results At the third day of culture polarized vs non-polarized cellular sub-populations were clearly established Biomaterials that showed more than 40% of polarized cells at the 3rd day of culture, subsequently showed an enhanced cell migration compared to biomaterials, where non-polarized cells predominated (p < 0 003) This trend continued untill the 7th day of culture (p < 0 003) The expression of vascular endothelial growth factor was enhanced in biomaterials where cell polarization predominated at the 7th day of culture (p = 0 001) Conclusions This model opens an interesting approach to understand osteoconductivity at a cellular level MSCs are promising in bone tissue engineering considering the strong angiogenic effect before differentiation occurs (C) 2010 European Association for Cranio-Maxillo-Facial Surgery
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL GROWTH-FACTOR; PLATELET-RICH PLASMA; MORPHOGENETIC PROTEIN-7; STROMAL CELLS; HYDROXYAPATITE; ANGIOGENESIS; bone substitutes; mesenchymal stem cells; green fluorescent protein; confocal scanning laser microscopy; cell migration; cell polarity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jul 2020 08:38 |
| Last Modified: | 06 Jul 2020 08:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23804 |
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